Beta-2-microglobulinFocal segmental glomerulosclerosisPurpose. After observing variation in the expression of the housekeeping gene B2M in cells of the urinary sediment during a study of candidate genes potentially involved in diabetic kidney disease (DKD), we hypothesized that B2M mRNA expression in ...
beta-2-microglobulin Immunoglobulin-like polypeptide (119aa), homologous with the constant region of Ig, that is found on the surfaces of most cells, associated non-covalently with Class I histocompatibility antigens. beta-actin One of many actin isoforms but found (like gamma-actin) predominantly...
Expression profiles of four housekeeping genes namely 尾-2 microglobulin ( B2M ), hypoxanthine-guanine phosphoribosyl transferase-1 ( HPRT1 ), 尾-actin ( ACTB ) and glyceraldehyde 3-phosphate dehydrogenase ( GAPDH ) were analyzed by RT-qPCR. The results were statistically analyzed to select ...
Human brain weight is correlated with expression of the ‘housekeeping genes’ beta‐2‐microglobulin (β2M) and TATA‐binding protein (TBP)[J] . P. J.Harrison,L. M.Laatikainen,E. M.Tunbridge,S. L.Eastwood.Neuropathology and Applied Neurobiology . 2010 (6)...
Results: Brain weight correlated with the 'raw' (i.e. non-normalized) data for two mRNAs, 尾2-microglobulin and TATA-binding protein, measured in cerebellum and hippocampus, respectively. In hippocampus, the geometric mean of three housekeeping gene transcripts also correlated with brain weight. ...
Reddy NR and Wilkie BN (2000): Quantitation of porcine cytokine and beta 2- microglobulin mRNA expression by reverse transcription polymerase chain reaction. J. Immunol. Methods. 233, 83-93.Reddy N R,Wilkie N. Quantitation of porcine cytokine and beta2-microglobulin mRNA expression by reverse ...
Data are represented as fold change to the control or LPS-treated group after normalization to the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and beta-2-microglobulin (B2M) housekeeping genes. Primers were designed using the NCBI Primer Blast program, and each primer set was validated for ...
As an internal control, glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and beta-2-microglobulin (B2M) housekeeping genes were used in the case of human samples, whereas β-actin was used in mice samples. Relative quantification was calculated using the 2−ΔΔCt formula. Results were ...