Nat. Methods 14, 865–868 (2017). This article introduces CITE-seq, which is one of the two essential assays for surface protein measurements. Article CAS PubMed Central PubMed Google Scholar Han, A., Glanville, J., Hansmann, L. & Davis, M. M. Linking T-cell receptor sequence to ...
Methods used in CFTR testing can be divided into two groups: those targeted at known mutations (ie, testing DNA samples for the presence or absence of specific mutation(s)) and scanning methods (ie, screening samples for any deviation from the standard sequence). These now include searching ...
Quality control for the raw reads involves the analysis of sequence quality, GC content, the presence of adaptors, overrepresentedk-mers and duplicated reads in order to detect sequencing errors, PCR artifacts or contaminations. Acceptable duplication,k-mer or GC content levels are experiment- and ...
In addition, tumors that exhibit high intratumoral heterogeneity can require alternative methods, such as collecting multiple biopsies per tumor [17]. Somatic variant callers identify single nucleotide variants (SNVs) from tumor and matched non-tumor DNA sequence data, such as whole genome, or more...
(multireads). Genomic multireads are primarily due to repetitive sequences or shared domains of paralogous genes. They normally account for a significant fraction of the mapping output when mapped onto the genome and should not be discarded. When the reference is the transcriptome, multi-mapping ...
parental samples, or by using proxy methods (for example, apparent linkage to parentally informative SNPs on NGS reads). For two rare coding variants observed in gnomAD, it is possible to estimate the likelihood that they are in cis (on the same allele), see https://gnomad.broadinstitute....
In the era of rationally designed synthetic biology, heterologous metabolites production, and other counter-nature engineering of cellular metabolism, we took a step back and recalled that ‘Mother(-Nature) knows best’. While still aiming at synthetic,
Our team particularly enjoyed the challenge of creating a moving graphic for the homepage that looks like a bold, bright, sequence of DNA. We used a black background to make the colors pop and captivate site visitors. One thing that stood out about this project was how closely we were able...
Billy Corgan is debatably the genre’s most popular yet polarizing spokesman, and at least two of their studio sequences (1993’s Siamese Dream and 1995’s Mellon Collie and the Infinite Sadness) embody alternative rock at its most artistically and commercially significant. Aside from Corgan’s ...
The sequence targeting shBest3-II was: 5′-GCAGATGAGAGGAAATTATTC-3′. Blocking of ion channels or ion pumps. Bafilomycin (BFA): Twelve hours after Best3V2_HA transfec- tion, HeLa cells were treated with BFA (2 nM) for 12 h. Anion channel blockers NPPB, DIDS and ...