TO-PRO-3 Stain Protocol NucGreen Dead 488 ReadyProbes Reagent Protocol for Fixed Cells BestProtocols: Annexin V Staining Protocol for Flow Cytometry BestProtocols: BrdU Staining Protocol for Flow Cytometry BestProtocols: Cell Preparation for Flow Cytometry Protocols BestPro...
TO-PRO-3 Stain Protocol NucGreen Dead 488 ReadyProbes Reagent Protocol for Fixed Cells BestProtocols: Annexin V Staining Protocol for Flow Cytometry BestProtocols: BrdU Staining Protocol for Flow Cytometry BestProtocols: Cell Preparation for Flow Cytometry Protocols BestProtoco...
In conclusion, we describe a reliable method to detect the activation of porcine platelets and therefore provide a useful tool for platelet flow cytometry in porcine models. Notably, the applied incubation protocol and medium, in which platelets are suspended, have major effects on antibody-binding ...
Scientists have to learn new experimental techniques all the time. However, being given a protocol from a labmate or taking your sample over to the core facility does not actually endow you with any background knowledge related to that technique. When it comes to flow cytometry—a technique t...
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1 and 2b). The selection of protocol steps depends on the volume and complexity of the source, other extracellular particles residing in the source, and BEV subsets of interest2. Considering that each protocol prepares BEVs with differential specificity (also referred to as purity) and efficiency...
Mobilization protocol consisted of subcutaneous injections of G-CSF (Neupogen, Amgen, Mississauga, Ontario, Canada) 5 μg/kg twice a day for 9 doses. Cytaphereses were initiated after the ninth injection using a continuous-flow blood separator (CobeSpectra, Cobe Laboratories, Lakewood, CO, USA...
A second approach adopted in multiple studies is to use laser capture microdissection to isolate cells of interest [69], [78] Additionally, flow cytometry has been successfully used to select specific cell subtypes, such as epithelial cells, in samples of dissociated, intact cells [76]. Finally...
BestProtocols: Annexin V Staining Protocol for Flow Cytometry BestProtocols: BrdU Staining Protocol for Flow Cytometry BestProtocols: Cell Preparation for Flow Cytometry Protocols BestProtocols: UltraComp Compensation Beads Protocols for Flow Cytometry BestProtocol...
BestProtocols: Annexin V Staining Protocol for Flow Cytometry BestProtocols: BrdU Staining Protocol for Flow Cytometry BestProtocols: Cell Preparation for Flow Cytometry Protocols BestProtocols: UltraComp Compensation Beads Protocols for Flow Cytometry BestProtocols: P...