在功能上,它通过与受体酪氨酸蛋白激酶 B (TrkB) 的相互作用,在促进神经发生、神经元分化和突触可塑性方面发挥关键作用 (Lu et al.,2014)。BDNF/TrkB 信号级联反应的激活随后触发转录因子 cAMP 反应元件结合蛋白 (CREB) 的磷酸化 (W...
应用Positive Control; Immunogen; SDS-PAGE; WB.如果需要有生物活性的蛋白,请参见活性蛋白。 规格:10μg 50μg 200μg 1mg 1g (保证有货万一缺货免费重做) 储存:避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。 脑源性神经营养因子(BDNF)重组蛋白的特性: ...
Invitrogen Anti-BDNF Polyclonal, Catalog # PA5-15198. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications. This antibody reacts with Human samples. Supplied as 400
图2|HG-9-91-01逆转了CUMS诱导的海马SIK2-CRTC1系统的功能障碍 随后,课题组研究了HG-9-91-01对BDNF信号通路的影响,WB结果显示:CUMS小鼠BDNF,pERK1/2,pAKT,pCREB的表达显著降低,而HG-9-91-01显著缓解了CUMS对海马BDNF信号级联通路的这种抑制作用。 图3|HG-9-91-01缓解了CUMS对海马BDNF通路的抑制作用 此...
4,TUNEL染色:S组未见明显的凋亡阳性细胞;相较于S组,M组脊髓前角灰质内有大量的凋亡细胞(p<0.05);HM组与M组比较凋亡细胞显著下降(p<0.05);与M组比较,IM组凋亡细胞显著增加(p<0.05);与HM相比,IHM组凋亡细胞增加(p<0.05). 5,WB:M组与S组比较,BDNF,TrkB,c-caspase3,Bax/Bcl-2表达均增加(p<0.05),而...
运动训练可明显增加SCI-TT/PBS组KCC2的表达量,其免疫强度和相对光密度值均高于SCI/PBS组、SCI/TrkB-IgG组和SCI-TT/TrkB-IgG组( P<0.05)。而SCI/TrkB-IgG组与SCI-TT/TrkB-IgG组差异无统计学意义( P>0.05)。 结论减重平板训练可通过BDNF-TrkB信号通路改善SCI大鼠的痉挛状态并促进损伤远端脊髓内KCC2的表达...
pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, al...
WB: Western blotting ELISA: Enzyme-linked immunosorbent assay TNF-α: Tumour necrosis factor α IL-1β: Interleukin 1 beta IL-6: Interleukin-6 Iba-1: Ionized calcium-binding adaptor molecule-1 LPS: Lipopolysaccharides BDNF: Brain-derived neurotrophic factor TrkB: Tyrosine kinase recepto...
to Park and Poo [126]) can directly influence late effects in synaptic plasticity, for instance, local protein synthesis, spine remodeling, or gene transcription.bBDNF as an instructor of synaptic plasticity. Glutamate and BDNF are released within a critical time window (bright blue) and TrkB ac...
TrkB-Fc/BDNF or IgG complexes were depleted from CM-E2 using agarose beads.g4T1BR5 cells were plated as in (b, right) and treated with vehicle, CM-OH, CM-E2 alone or in combination with 1 µm ANA-12. Graph shows number of mammospheres at day 10.h. Left: WB shows TrkB and...