High-performance liquid chromatography (HPLC) is a sophisticated technique. Chromatographers must not forget the basics of method development to reap the full benefits in practice. Obvious parameters to optimize include the choice of chromatographic column, mobile phase, injection volume, flow rate, ...
Basic HPLC System Components Isocratic vs. Gradient Elution Modes Screening Gradient – Powerful for Method Development Available Detectors for HPLC HPLC Columns The Chemistry of Bonded Phases The Phenomenon of Hydrophobic Retention Learn more about HPLC Products ...
This experiment was the beginning of the HPLC development journey, although it took another 30 years before using pumps to push a liquid phase through the packed column. Today, laboratories worldwide use HPLC to identify and quantify non- and semi-volatile chemical components in...
Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, TX Florian L. Muller Functional Genomics Core, Children's Hospital Oakland Research Institute, Oakland, CA Kenneth B. Beckman ...
The advent of novel 18F-labeling methods has just begun. The introduction of Si-18F [5–7], B-18F [5,6] and Al-18F [8–12] radiochemistry has set the foundation for the development of novel radiotracers and has the potential to reshape the landscape of 18F-radiochemistry if some ...
1 Overview of HPLC HPLC is an abbreviation for High Performance Liquid Chromatography. "Chromatography" is a technique for separation, "chromatogram" is the result of chromatography, and "chromatograph" is the instrument used to conduct chromatography. Among the various technologies developed for chrom...
New York: John Wiley & Sons, Inc., 1997. Volume 1, 2. p. 21-58.Snyder LR, Kirkland JJ, Gljach JL (1997a,b) Basics of separation. p. 51- 56 (a), Optimizing the reversed-phase separation. p. 309 (b). In: Practi- cal HPLC Method Development, Wiley, New York....
Snyder LR, Kirkland JJ, Gljach JL (1997a,b) Basics of separation. p. 51- 56 (a), Optimizing the reversed-phase separation. p. 309 (b). In: Practi- cal HPLC Method Development, Wiley, New York.SNYDER, L. R.; KIRKLAND, J. J. & GLAJCH, J. L. Basics of Separation. In. ...
超高效液相色谱分析 超高效液相色谱分析 (UHPLC) 法可从基于 <2 µm 固定相颗粒开始,运行时的工作压力介于 600-1200 bar 之间,流速介于 0.2 – 0.7 mL/min 之间。 与标准 HPLC 系统相比,压力范围更大,可实现更优的分辨率和灵敏度、更高的通量以及更少的溶剂用量。UHPLC ...