Comparison of Preparation Methods of Bacterial Cell-Mineral Aggregates for SEM Imaging and Analysis Using the Model System of Acidovorax sp. BoFeN1Cell-mineral aggregatescritical point dryingextracellular polymeric substancesfreeze dryingpreparation artifacts...
4. Raman Spectroscopy The Raman spectrum offers bacterial-specific molecular fingerprints which can be used for analysis. [25,26] 5. Optical Microscopy Rapid and non-destructive visualization and characterization of cells with minimal sample preparation. [27,28] 6. Confocal Laser Scanning Microscopy ...
Frequency calibration for heptapeptides binding to the lethal bacterial strainMSI001.aFlowchart for sequence data processing and heptapeptide normalization to the phage library.bSequence composition of recovered samples and phage library inputs. Peptides for analysis were acquired by removing the sequences...
The size, morphology, and structure of the bacterial nanocellulose samples produced byBacillussp.strain SEE-3 coated with gold using a sputter coater (SPI-Module) and were analysed by a scanning electron microscope (SEM) “JSM-5500 LV; JEOL, Ltd- Japan; by using high vaccum mode operating at...
Quantitative real-time PCR analysis The primers used for real-time PCR quantification of total bacteria, fungi, protozoa, and methanogens are listed in Table S1. Standard preparation was carried out by monoclonal sequencing of the PCR-recovered products after retrieval of the target gene. Production...
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We compare this approach with: (1) standard preparation with glutaraldehyde fixation, ethanol dehydration, and CPD before CSEM, (2) cryo-sputter preparation of rapidly frozen biofilm in hydrated state (cryo-SEM), and (3) in situ observation without any sample pretreatment in environmental SEM. ...
We also use optional cookies for advertising, personalisation of content, usage analysis, and social media. By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with...
extending intogp41, which encode the terminase small subunit (TerS) and large subunit (TerL) (Fig.1eandSupplementary Sequences). Similar results were obtained for the cyclase-bound RNA generated during ΦNM1γ6 infection (Extended Data Fig.2handSupplementary Sequences). Northern blot analysis of...
Analysis of variance (ANOVA) was followed by a post-hoc evaluation with Tukey’s Studentized Range (HSD) test. A p value < 0.05 was deemed statistically significant. The data present the means ± Standard Error of the Mean (SEM). 3. Results 3.1. Bacterial Isolates P1 to P8 belong to ...