Comparison of Preparation Methods of Bacterial Cell-Mineral Aggregates for SEM Imaging and Analysis Using the Model System of Acidovorax sp. BoFeN1Cell-mineral aggregatescritical point dryingextracellular polymeric substancesfreeze dryingpreparation artifacts...
SampleTail moment ± SEM FibroblastsNeuroblastoma cells Platinum control (unstructured) 1.32 ± 0.24 – Platinum fractal roughness 1.41 ± 0.28 1.19 ± 0.18 Silicon control (unstructured) 1.4 ± 0.34 1.23 ± 0.18 Silicon spike structured 1.48 ± 0.52 1.09 ± 0.17 Also geometrically defined groove str...
Effectively killing pathogenic bacteria is key for the treatment of sepsis. Although various anti-infective drugs have been used for the treatment of sepsis, the therapeutic effect is largely limited by the lack of a specific bacterium-targeting delivery system. This study aimed to develop antibacteri...
We compare this approach with: (1) standard preparation with glutaraldehyde fixation, ethanol dehydration, and CPD before CSEM, (2) cryo-sputter preparation of rapidly frozen biofilm in hydrated state (cryo-SEM), and (3) in situ observation without any sample pretreatment in environmental SEM. ...
Scanning electron microscopy (SEM) images of the sonicated bacterial nanocellulose sample produced byBacillussp.strain SEE-3 on Cantaloupe juice after treatment process (Fig.4G,H) revealed crystalline clusters and spherical particles. Results demonstrated that, ultrasonic treatment produced small size partic...
SEM, standard error of means. T, the effect of treatments; D, the effect of ensiling periods; T × D, the interaction of treatment and ensiling periods Full size image The chemical composition of WPCS fermented for 90 d is reported in Table 1. All 3 inoculants increased the DM content...
Understanding the interactions between EPF, host insects, and gut microbiota will be beneficial for developing novel biological pest control strategies. Methods Insect rearing, antibiotic treatment, and sample preparation Helicoverpa armigera larvae were maintained at 28 ± 1 °C and 70% relative ...
Time axes for individual cells were aligned such that τfiss=0min. Error bars represent SEM. The dashed blue and red lines are linear fits to the pre- and fission periods, with slopes 10.6×10−3 and 7.9×10−3μm2/min. (H) Similar to (G), but for the mother cell areas. The...
Transmission and scanning electron microscopes (TEM and SEM, respectively) can also be combined with cellular dyes in order to obtain higher resolutions for both qualitative and quantitative assessment of the targeted organisms. For example, a CARD-based approach using gold nanoparticles (GOLD-FISH) ...
based on the mask types and duration of mask usage. Mean + standard error of the mean (SEM). The pairedt-test and Student’st-test were used for statistical analyses. *P < 0.05; **P < 0.001. Full size image Microbial counts on the face-side and outer-side of masks...