The TRIzol® Max™ Bacterial RNA Isolation Kit provides a simple and reliable method to improve the isolation of intact total RNA from gram-positive and gram-negative bacteria. The kit utilizes both the Max™ Bacterial Enhancement Reagent and TRIzol® Reagent to inactivate endogenous RNases ...
E.Z.N.A.® Bacterial RNA Kit 从处于指数生长期的细菌培养物中提取RNA 该试剂盒采用硅胶柱纯化方式,适合于从各种来源的细菌(革兰氏阳性或阴性菌等)培养液中快速简单地提取RNA。一次可以处理3ml正处于指数生长期的细菌培养物(细菌数量:1x109)。纯化的RNA产物可直接用于RT-PCR,Northern杂交等实验。完成一次抽提...
目前该公司的产品多达700余种,提供一流的样品制备试剂盒,应用于基因组和蛋白组等不同的领域。其产品主要覆盖包括各种标本类型的DNA,RNA和蛋白质的纯化富集,从活检样本(包括尿液,血浆,血清和血液)中纯化RNA和DNA,全系列的PCR试剂、缓冲液、分子量ladder和塑料过滤装置。
细胞表面蛋白分离试剂盒(Bacterial Surface Protein Isolation Kit)操作步骤 (一)获得目的基因 1、通过PCR方法:以含目的基因的克隆质粒为模板,按基因序列设计一对引物(在上游和下游引物分别引入不同的酶切位点),PCR循环获得所需基因片段。 2、通过RT-PCR方法:用TRIzol法从细胞或组织中提取总RNA,以mRNA为模板,逆转录...
RNA was isolated with the Agilent Total RNA Isolation Mini Kit following the detailed protocol in Appendix A, or by a silica-based method (QIAGEN RNeasy and RNase-free DNase Set, QIAGEN, Valencia, CA). RNA quality was determined via the Prokaryotic Total RNA Nano asasy from the RNA 6000 ...
(not included in the kit) provide a set of external rna controls that enable performance assessment for dynamic range, lower limit of detection, and fold-change response for gene expression experiments. for detailed information, refer ...
A Method of RNA Isolation of Bacterial from Infected Mammalian Cells一种从感染的培养细胞中分离细菌RNA的方法志贺氏菌胞内细菌RNA提取冷酸酚法病原菌全基因组表达谱研究是阐明其致病机理的必要的基础 ,已经成为当前生命科学领域的热点和重点 ;然而由于难于从感染的组织中快速固定并分离细菌RNA ,从而极大的制约了...
The total RNA was prepared using the RNA Extraction Kit (QIAGEN, Germany) and rRNA was removed using Ribo-Zero rRNA Removal Kit (Epicentre, United States), following the manufacturer’s protocol. Sequencing libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit for ...
then 5 µl phenol and 50 µl ethanol mixed in before re-pelleting. Phenol-pellets were stored at −80 °C. RNA was purified from phenol-pellets using the TRIzol Max Bacterial RNA Isolation Kit (Thermo Fisher) and subsequently DNase treated using the TURBO DNase Kit (Invitrogen)...
RNA extraction. The total RNA was prepared using the RNA Extraction Kit (QIAGEN, Germany) and rRNA was removed using Ribo-Zero rRNA Removal Kit (Epicentre, United States), following the manufacturer’s protocol. Sequencing libraries were generated using the NEBNext Ultra Directional RNA Library ...