DNA extraction from soil samples is a critical step for molecular biology analyses. The present study compared the efficiency of two DNA isolation methods from non-polluted and polluted soils with or without the presence of a plant. Both applied methods used chemical and physical lyses, but ...
Objective To compare four methods of bacterial genomic DNA extraction from Sprague-Dawley rat fecal samples.Methods Sixteen fecal samples with 106 cfu S.aureus and 4 fecal samples without S.aureus were equally divided into four groups.Bacterial genomic DNA were extracted by following methods:(1) Se...
Method performance was assessed by comparing ease of use, DNA yield, DNA quality, PCR amplicon yield, and the detection of bacterial taxa by 16S rRNA amplicon sequencing. Results All three DNA extraction methods yielded DNA of sufficient quantity and quality to perform quantitative PCR and 16S ...
Boiling, Freezing-thawing and Simple Chemical Extraction Methods are Comparable to Costly Kit-based Bacterial DNA Extraction Methods as Evidenced by Conven... A Shehata,A Hershan 被引量: 0发表: 2016年 Stability of Human Immunodeficiency Virus RNA in Blood Specimens as Measured by a Commercial ...
DNA-based monitoring of pathogens in aerosol samples requires extraction methods that provide high recovery of DNA. To identify a suitable method, we evaluated six DNA extraction methods for recovery of target-specific DNA from samples with four bacterial agents at low abundance (<10,000 genome cop...
The objective of this study was to evaluate the most effective method of DNA extraction of oral mouthwash samples for use in microbiome studies that utilize next generation sequencing (NGS). Eight enzymatic and mechanical DNA extraction methods were test
However, besides inherent limitations of primer-based PCR methods such as the presence of Taq DNA polymerase inhibitors [7], [8], [9] the human genome itself may interfere with the detection and diagnosis of pathogens resulting in significant signal-to-noise problems [1]. Among other factors,...
Separation of bacterial DNA from human DNA in clinical samples may have an important impact on downstream applications, involving microbial diagnostic syst... VG Conrads - 《Anaerobe》 被引量: 74发表: 2010年 New methods for selective isolation of bacterial DNA from human clinical specimens Separatio...
order to optimize the DNA extraction from basaltic rocks, sterilized basalts with different particle sizes and chemically synthesized goethite were inoculated with an iron oxidizer Marinobacter aquaeolei and an extreme halophilic archaeon Halobaculum gomorrense respectively, and extracted with several methods...
Microbial community profiling based on 16S rRNA genes is fraught with bias created by DNA extraction methods, competitive DNA (human or otherwise), primers used during PCR amplification, the sequencing platform used, depth of coverage, and bioinformatics [20, 27], to name just a few. Depending...