Clearly, the choice of DNA extraction protocol depends on soil type. We found, however, that for the analysis of indigenous soil bacterial communities the bead-beating procedure was appropriate because it is fast, reproducible, and gives very pure DNA of relatively high molecular weight. And very...
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Objective To compare four methods of bacterial genomic DNA extraction from Sprague-Dawley rat fecal samples.Methods Sixteen fecal samples with 106 cfu S.aureus and 4 fecal samples without S.aureus were equally divided into four groups.Bacterial genomic DNA were extracted by following methods:(1) Se...
DNA extraction DNA was extracted from frozen thrombotic material using the Illustra DNA Extraction Kit BACC3 (GE Healthcare) following the protocol, with minor modifications. Mechanical tissue disaggregation was specifically designed for preparing thrombotic material for DNA extraction. DNA purity and conce...
To identify the polymorphisms associated with TCS resistance evolution, we sequenced 13 TRMs obtained from day 7 (4× MIC) to day 10 (16× MIC), exhibiting high resistance to TCS. Bacterial DNA was extracted using genomic DNA (gDNA) extraction kit (TIANGEN, China) following the manufacturer’...
Contaminating endogenous DNA from DNA extraction and processing kits produced CT values of 28–30 in no template controls using universal rRNA gene primers. Samples containing low bacterial biomass and high levels of competing human DNA, such as intestinal submucosal or peripheral blood samples, often...
Contaminating endogenous DNA from DNA extraction and processing kits produced CT values of 28–30 in no template controls using universal rRNA gene primers. Samples containing low bacterial biomass and high levels of competing human DNA, such as intestinal submucosal or peripheral blood samples, often...
Meier. We thank Rebecca Hallas, Rachel Leihy, and Katie Moon who provided assistance in the field, and Paul Czechowski for performing the DNA extractions of dataset 10 samples. We particularly thank Fourie Joubert and Johann Swart for tirelessly maintaining the University of Pretoria servers....
DNA extraction Total bacterial DNA was extracted from each soil sample using the Power Soil DNA Isolation Kit (MO BIO Laboratories), according to the manufacturer’s protocol. DNA quality and quantity were assessed by the ratios of 260 nm/280 nm and 260 nm/230 nm, respectively. Then...
DNA extraction included an initial mechanical lysis step using FastPrep24 instrument (MP Biomedicals Europe) at 6.5 m s−1for 90 s and afterwards was accomplished using E.Z.N.A Soil DNA Extraction kit (Omega Bio-Tek, USA) following the manufacturer’s protocol. ...