Here we present a modified version of the Marmur procedure (Marmur, 1961) for extraction, isolation and purification of bacterial DNA. The protocol is effective in providing large amounts of high quality and high molecular weight genomic DNA from Gram-negative and Gram-positive bacteria, suitable...
本试剂盒抽提的DNA长度可达100kb以上。 六,储存常温储存一年。 七, 问答 Q-1基因组DNA得率低或无基因组DNA A-1一般情况下,从100µl-1ml过夜培养的大肠杆菌中,可以提取1-10µg的基因组DNA,推荐的菌液量是用500µl的过夜培养的浓菌液。我们不建议用过多的菌液,可能导致溶液不足而降低所提DNA的...
DNA extraction DNA was extracted from frozen thrombotic material using the Illustra DNA Extraction Kit BACC3 (GE Healthcare) following the protocol, with minor modifications. Mechanical tissue disaggregation was specifically designed for preparing thrombotic material for DNA extraction. DNA purity and conce...
Contaminating endogenous DNA from DNA extraction and processing kits produced CT values of 28–30 in no template controls using universal rRNA gene primers. Samples containing low bacterial biomass and high levels of competing human DNA, such as intestinal submucosal or peripheral blood samples, often...
To identify the polymorphisms associated with TCS resistance evolution, we sequenced 13 TRMs obtained from day 7 (4× MIC) to day 10 (16× MIC), exhibiting high resistance to TCS. Bacterial DNA was extracted using genomic DNA (gDNA) extraction kit (TIANGEN, China) following the manufacturer’...
For DNA extraction, we found that, as expected, kits optimized for soil were the best for soil, though each still included a distinct "fingerprint" of its own biases. Plant samples were less clear, with different species having different "best" options. For 16S rRNA gene amplification, we ...
Because of our permissive approach to assess bacterial diversity, e.g., the ticks used in this study were not disinfected prior to DNA extraction, the prevalence of these new bacterial associations with R. microplus needs to be confirmed. However, it is relevant to note that S. dysgalactiae ...
13527) [22] were grown as described in the literature [20,21,22]. The cells were harvested in the late exponential phase. DNA was extracted from the cell pellet using the PowerLyser® PowerSoil® DNA extraction kit (MoBio, Carlsbad, CA, USA) according to the manufacturer’s protocol....
Surprisingly, we found that in native cells RsAgo associates with a complex population of both small RNA and small DNA species. Analyses of nucleic acid partners of RsAgo suggest that associated small RNAs are generated by broad sampling of the bacterial transcriptome, and formation of small DNA...
Resulting colonies were purified on agar containing kanamycin, and plasmids were extracted using the AccuPrep DNA extraction kit (Bioneer, Alameda, CA). Sequencing was performed using transposon-specific primers (38). Capillary sequencing was performed using Big Dye Terminator V3 on an ABI 3730xl ...