2007. Analysis of DNA processing reactions in bacterial conjugation by using suicide oligonucleotides. EMBO J. 26: 3847-3857.Gonzalez-Perez B, Lucas M, Cooke LA, Vyle JS, de la Cruz F, Moncalian G. 2007. Analysis of DNA processing reactions in bacterial conjugation by using suicide ...
One is the conjugation systems of Gram-negative and Gram-positive bacteria that mediate the spread of plasmids among bacterial populations. A second subgroup involves newly identified systems that export and/or import DNA from the extracellular milieu, independent of contact with a target cell. The ...
Acquired by direct uptake (transformation), the action of viruses (transduction), or as transmissible plasmids (conjugation), traits encoded by horizontally acquired DNA can be beneficial2,3,4,5. For example, sequences encoding virulence factors, cell surface modifications and antibiotic resistance, ...
marina wild-type strain and plating the mixture on 1/2 SW-LB-DAP agar plates for conjugation transfer. Following incubation at 25 °C for 12–48 h, the colonies were diluted and streaked on Zobell 2216E plates containing erythromycin to select single colonies. PCR verification was ...
Among the bacteria, plasmids are mostly transferred horizontally by conjugation process. Plasmid replication process can be divided into three stages: initiation, elongation, and termination. The process involves DNA helicase I, DNA gyrase, DNA polymerase III, endonuclease, and ligase.Plasmids contain ...
Typhimurium by conjugation. S. Typhimurium transconjugants clones that have integrated the suicide plasmid were selected on solid M9 minimal medium supplemented with 0.2% of glucose and 50 µg/mL of kanamycin. pSW-2, purified from LT2-WT strain, was then introduced into transconjugants by ...
biotin-streptavidin conjugation, and in-situ polymerization. 2) Electrostatic contact, physical extrusion, self-assembly, and layer-by-layer techniques are the principal examples of physical interaction. 3) The two primary biological interactions that result in outcomes that are visible on the surface ...
The discovery of bacterial conjugation in 1946 was hailed by Salvador Luria in 1947 as “probably among the most fundamental advances in the whole history of bacteriological science,” even before the most basic facets of the process were known. This proved prophetic and even an understatement...
Wartel for helping in establishing high-throughput conjugation; E. van Nimwegen for E. coli SC402; and V. Mutalik and B. Adler for Ffm and Br60 phages. This work was supported by EMBL and the Sofja Kovaleskaja Award of the Alexander von Humboldt Foundation. K.M. and A.M. were ...
Transformation ofR. pomeroyithrough conjugation Reporter plasmids were transformed intoR. pomeroyiDSS-3 through a triparental conjugation method, which was found to be ideal due to the large sizes of our reporter plasmids (up to 7.974 kb). Overnight liquid culture of wild-typeR. pomeroyiwas pr...