the tube with a pipet tip (do NOT let the frozen cell thaw) and spread on an LB Agar plate with antibiotic. Culture overnight in the 37 o C incubator and store the new plate wrapped in parafilm in the fridge (4 o C). Materials (autoclave all media and glassware before using) ...
Deepa RajuHospital for Sick ChildrenDavid RizzutiHospital for Sick ChildrenNicola L. JonesHospital for Sick ChildrenMethods Mol BiolMethods in Molecular BiologyRaju, D., Rizzuti, D., and Jones N.L. (2012c). Cell culture-based assays to test for bacterial adherence and internalization. Methods ...
To obtain high transformation efficiency, it is crucial that cell growth is in mid-log phase at the time of harvest—which generally occurs at OD600 between 0.4 and 0.9; the optimal value depends on the culture volume, strain...
Oocytes were injected with 2 ng of WT or mutantdspEcRNA and incubated in bath ND96 saline for 15 h. Surface-exposed proteins were biotinylated and purified using the Pierce Cell Surface Protein Biotinylation and Isolation Kit (Thermo Fisher), following the manufacturer’s protocol with some ...
1a) in 14 ml culture tubes, yielding spheroids typically 0.2–2 mm in diameter after 3 days of culture (Fig. 1b). We also tested whether our protocol for spheroid cultivation would also work for genetically modified strains of K. rhaeticus containing plasmids expressing transgenes. We ...
To obtain high transformation efficiency, it is crucial that cell growth is in mid-log phase at the time of harvest—which generally occurs at OD600 between 0.4 and 0.9; the optimal value depends on the culture volume, strain...
Aliquots (1.5, 1.0, and 0.5 ml) of the enrichment culture were sampled at the early-, mid-, and late-exponential growth phases, respectively, and centrifuged. DNA in the cell pellets was extracted using Qiagen DNeasy Blood & Tissue Kit according to the manufacturer’s instructions (Nihon eid...
The mixed sample was then lysed by mechanical disruption with a bead beater (BioSpec Products) for 2 min at 20°C), followed by extraction with a QIAamp DNA Stool Mini Kit (Qiagen) using its protocol for isolation of DNA for pathogen detection. Lysis temperature was increased to 95°C ...
(DP) are shown in Table3. The comparison of absolute values concerning these MWD indicators with other authors should always be conducted carefully as they strongly depend on the selected protocol for sample preparation and the overall metrological approach (Potthast et al.2015). Additionally, ...
Chen Z-Y, Hsieh Y-M, Huang C-C, Tsai C-C. Inhibitory effects of probiotic lactobacillus on the growth of human colonic carcinoma cell line HT-29. Molecules. 2017;22:107.https://doi.org/10.3390/molecules22010107. ArticleCASPubMed CentralGoogle Scholar ...