Na+H+WallertandProvostLabExcelwithhardworkandinquirySept051BacterialCultureProtocolforPlasmidDNABacterialCultures:Therearetypi..
Large oligonucleotide pools demonstrated high potential of large-scale data storage in test tube, meanwhile, living cell with high fidelity in information replication. Here we show a mixed culture of bacterial cells carrying a large oligo pool that was assembled in a high-copy-number plasmid was ...
To obtain high transformation efficiency, it is crucial that cell growth is in mid-log phase at the time of harvest—which generally occurs at OD600 between 0.4 and 0.9; the optimal value depends on the culture volume, strain...
Oocytes were injected with 2 ng of WT or mutantdspEcRNA and incubated in bath ND96 saline for 15 h. Surface-exposed proteins were biotinylated and purified using the Pierce Cell Surface Protein Biotinylation and Isolation Kit (Thermo Fisher), following the manufacturer’s protocol with some...
Aliquots (1.5, 1.0, and 0.5 ml) of the enrichment culture were sampled at the early-, mid-, and late-exponential growth phases, respectively, and centrifuged. DNA in the cell pellets was extracted using Qiagen DNeasy Blood & Tissue Kit according to the manufacturer’s instructions (Nihon eid...
In this report, the real-time cell-binding assay (RT-CBA) technology LigandTracer®, originally designed for mammalian cell culture, was extended to cover Gram-positive and Gram-negative bacteria. This required the development of new immobilization methods for bacteria, since LigandTracer depends ...
The mixed sample was then lysed by mechanical disruption with a bead beater (BioSpec Products) for 2 min at 20°C), followed by extraction with a QIAamp DNA Stool Mini Kit (Qiagen) using its protocol for isolation of DNA for pathogen detection. Lysis temperature was increased to 95°C ...
lactis in batch and fed-batch culture. Microb Cell Fact. 2007;6:16. https://doi.org/10.1186/1475-2859-6-16. Even S, Lindley ND, Cocaign-Bousquet M. Molecular physiology of sugar catabolism in Lactococcus lactis IL1403. J Bacteriol. 2001;183:3817–24. https://doi.org/10.1128/JB....
subtilis spores were incubated on droplets (30 µl) at room temperature using the following protocol: 2x glycine (50 mM) in PBS for 5 min; blocking solution (0.5% fish gelatin, 0.5% bovine serum albumin [protease free; T844.1; Carl Roth GmbH, Germany], and 0.01% Tween 20 [...
Biofilms serve as crucial cues for settlement and metamorphosis in marine invertebrates. Within bacterial systems, c-di-GMP functions as a pivotal signaling molecule regulating both biofilm formation and dispersion. However, the molecular mechanism of ho