Na+H+WallertandProvostLabExcelwithhardworkandinquirySept051BacterialCultureProtocolforPlasmidDNABacterialCultures:Therearetypicallytworeasonsforcultur..
Nicola L JonesMethods Mol BiolMethods in Molecular BiologyRaju, D., Rizzuti, D., and Jones N.L. (2012b). Cell culture assays to evaluate bacterial toxicity and virulence. Methods Mol Biol. 921, 77-88.Raju D, Rizzuti D, Jones NL. Cell culture assays to evaluate bacterial toxicity and ...
Oocytes were injected with 2 ng of WT or mutantdspEcRNA and incubated in bath ND96 saline for 15 h. Surface-exposed proteins were biotinylated and purified using the Pierce Cell Surface Protein Biotinylation and Isolation Kit (Thermo Fisher), following the manufacturer’s protocol with some ...
To obtain high transformation efficiency, it is crucial that cell growth is in mid-log phase at the time of harvest—which generally occurs at OD600 between 0.4 and 0.9; the optimal value depends on the culture volume, strain...
1a) in 14 ml culture tubes, yielding spheroids typically 0.2–2 mm in diameter after 3 days of culture (Fig. 1b). We also tested whether our protocol for spheroid cultivation would also work for genetically modified strains of K. rhaeticus containing plasmids expressing transgenes. We ...
Aliquots (1.5, 1.0, and 0.5 ml) of the enrichment culture were sampled at the early-, mid-, and late-exponential growth phases, respectively, and centrifuged. DNA in the cell pellets was extracted using Qiagen DNeasy Blood & Tissue Kit according to the manufacturer’s instructions (Nihon eid...
Ceftriaxone compared with penicillin G for the treatment of neurosyphilis: study protocol for a multicenter randomized controlled trial Trials, 23 (2022), 10.1186/s13063-022-06769-w Google Scholar 115 J.C. Tsai, Y.H. Lin, P.L. Lu, et al. Comparison of serological response to doxycycline ...
The mixed sample was then lysed by mechanical disruption with a bead beater (BioSpec Products) for 2 min at 20°C), followed by extraction with a QIAamp DNA Stool Mini Kit (Qiagen) using its protocol for isolation of DNA for pathogen detection. Lysis temperature was increased to 95°C ...
Biofilms serve as crucial cues for settlement and metamorphosis in marine invertebrates. Within bacterial systems, c-di-GMP functions as a pivotal signaling molecule regulating both biofilm formation and dispersion. However, the molecular mechanism of ho
subtilis spores were incubated on droplets (30 µl) at room temperature using the following protocol: 2x glycine (50 mM) in PBS for 5 min; blocking solution (0.5% fish gelatin, 0.5% bovine serum albumin [protease free; T844.1; Carl Roth GmbH, Germany], and 0.01% Tween 20 [...