Single-cellSingle-moleculeDuring the past decades, several methods (e.g., electron microscopy, flow chamber experiments, surface chemical analysis, surface charge and surface hydrophobicity measurements) have been developed to investigate the mechanisms controlling the adhesion of microbial cells to other ...
aSchematic diagram of DLL mechanism. N1 and N2 were two subdomains of Cna that wrapped collagen molecules (gray) through conformational changes.bSchematic diagram of bacteria–cell adhesion at the single-cell level.\({k}_{{{\rm{a}}}+}\)denoted the association rate whereas\({k}_{{{\rm{...
Insight into the mechanisms underlying bacterial adhesion is critical to the formulation of membrane biofouling control strategies. Using AFM-based single-cell force spectroscopy, we investigated the interaction between Pseudomonas fluorescens, a biofilm-forming bacterium, and polysulfone (PSF) ultrafiltration...
Many bacterial adhesins function as lectins and the interactions between bacterial lectins and host cell carbohydrates are among the best-characterized attachment processes. Hallmark examples of carbohydrate recognition include Pseudomonas aeruginosa, Haemophilus influenzae, and Streptococcus pneumoniae adhesion in...
We use σ = W to probe ordering at the single-cell level (σ → 0 gives sharp Li × W rectangles). The integrated cell ‘mass’ is preserved as σ varies. [Note that the functional form of fi we use is required to account for the non-spherical, rod-like shape of ...
coli decreased when increasing the surface free energy of the polymer, while L. acidophilus showed just the opposite behaviour. The comparative results indicated that the adhesion of BHK and MDBK cell was lowest on the most hydrophilic PHEMA surface and highest on the most hydrophobic PP surface....
2.1.1. Adhesion Bacterial adhesion to biotic or abiotic surfaces is the initial step of biofilm formation. The process of adhesion can be either reversible or irreversible [26]. Reversible attachment is a temporary adhesion of the planktonic bacterial cell onto a substratum with weak nonspecific bon...
vesicles and vacuoles are surrounded by a single biomembrane sheet. Mitochondria and the nucleus are surrounded by two membrane layers. Finally, the membrane regulates the flow of materials into and out of the cell, mediates intercellular communication, contacts and adhesion, and performs a multitude...
Cell wall hydrophobicity was measured as the contact angle of water on a bacterial layer collected on a microfilter. The contact angles ranged from 15 to 70 degrees. This method was compared with procedures based upon adhesion to hexadecane and with the partition of cells in a polyethylene ...
At the level of a single cell, external mechanical influences on bacterial growth, including fluid flows and confinements, have been extensively investigated3,12,13. Moreover, different models for the growth kinetics of individual bacteria14,15,16,17,18describe cell division events as regulated tow...