Clemons W M Jr,et al.Structure of a bacterial 30S ribosomal subunit at 5. 5 resolution. Nature . 1999Clemons, WM, Jr., May, JLC, Wimberly, BT, McCutcheon, JP, Capel, MS, and Ramakrishnan, V (1999). "Structure of a bacterial 30S ribosomal subunit at 5.5 A resolution." Nature (...
The 30S ribosomal subunit binds messenger RNA and the anticodon stem-loop of transfer RNA during protein synthesis. A crystallographic analysis of the structure of the subunit from the bacterium Thermus thermophilus is presented. At a resolution of 5.5 Å, the phosphate backbone of the ribosoma...
Binding of cf-labeled Api88 (red), Api137 (gray), and Onc112 (black) to purified 70S ribosome (circles, left), the 50S subunit (squares, right) and the 30S subunit (triangle, right) used to calculate dissociation constants (Kd, table) compared to previously reported Kdvalues using crude...
Translation initiation has consequently become the most highly regulated step of protein biosynthesis and, in bacteria, relies on characteristics of both the 30S ribosomal subunit and the mRNA [68]. Cells control the rate of ribosome-dependent protein synthesis through several mechanisms during ...
In the tertiary structure of the 30S ribosomal subu- nit, h41 locates at the top of the structure (the head) and protrudes to the exterior of the subunit27,28, potentially providing a direct binding site for RNase I. This direct interaction model is also sup- ported by the fact that ...
Tigecyclineis a glycylglycine derivative of minocycline that has increased affinity for the 30S ribosomal subunit and lower minimum inhibitory concentrations against many gram-positive and gram-negative organisms compared with older tetracyclines. It also has decreased susceptibility to resistance mechanisms af...
Materials and methods; Identification of protein Y; Isolation and purification of protein Y; Mass spectrometry of protein Y; Binding of protein Y to ribosomal particles; Exposure of protein Y on the surface of the 30S subunit and the 70S ribosome; Discussion....
During assembly, ribosomal particles in bacteria fold according to energy landscapes comprised of multiple parallel pathways. Cryo-electron microscopy studies have identified a critical maturation step that occurs during the late assembly stages of the 50S subunit in Bacillus subtilis. This step acts as...
and 30S ribosomal protein RpsI.205In addition tohipBA, several other TA modules have been associated with persister formation. These includerelBE(toxin RelE),206,207mazEF(toxin MazF),208dinJ-yafQ(toxin YafQ),209,210mqsRA(toxin MqsR),211,212ccdAB(toxin CcdB),213tisAB(toxin TisB),214,21...
The initiation of bacterial translation involves the tightly regulated joining of the 50S ribosomal subunit to an initiator transfer RNA (fMet-tRNAfMet)-containing 30S ribosomal initiation complex to form a 70S initiation complex, which subsequently matures into a 70S elongation-competent complex. Rapid...