Recalcitrant infections are often caused by dormant bacteria, including persisters and viable but nonculturable cells. Here, the authors explore how composition and structure of protein aggregates affect dormancy and exit from this state. Celien Bollen ...
Stem cells are foundin most tissues throughout the body, and each type does different things. With the ability to separate into numerous cell types, mesenchymal stem cells have multipotent properties, making them attractive for clinical applications. During the Mayo Clinic-sanctioned research on the ...
For quantitative analysis of cell uptake, the cells were digested and collected for flow cytometry analysis. In vivo bacterial clearance: One hundred microliters of blood from each mouse treated with EcN, PLCB, and CMCB as described in the section of Blood Reservation Study was withdrawn at 1.5...
Degradation of casein, the major milk protein, is dependent on a cell wall-associated proteinase; this protease hydrolyzes casein into oligopeptides of varied sizes that in turn enter the cell by different transport systems. Inside, peptides are further degraded into amino acids for microbial metabol...
Access rates to water and sanitation in West and Central Africa are among the lowest in the world. Out of 24 countries in the region, not a single one is on track to meet the MDG target for sanitation. This is the underlying cause for cholera outbreaks in West and Central Africa. Althou...
This electron microscope image reveals the bacteria living in a tumor cell. Credit: Weizmann Institute of Science Cancer cells are comfy havens for bacteria. That conclusion arises from a rigorous study of over 1,000 tumor samples of different human cancers. The study, headed by researchers at ...
By nature, humans are inquisitive animals: this is the foundation of scientific investigation. However, we sometimes have difficulty in comprehending, and therefore interpreting, the data; this is especially true when working with systems much different
of HSPG (Supplementary Fig.1), which showed that HSPG was highly expressed on three colon tumor cell lines (mouse CT-26, MC-38 and human HCT-116), compared with the relatively low expression in the breast cancer cell line, MCF-7. Next, we incubated the tumor cells with EGFP-expressed ...
Furthermore, we find Salmonella Typhimurium does not exhibit biased motion towards the cells and show that the search time statistics is consistent with a random search strategy. Our results indicate that in vitro localization of host cells, and also cell infection, are random processes, not ...
The presence of poly- and oligo-saccharides on the surface of microbial cells constitutes the base of using boronate affinity to capture bacteria [[16], [17], [18]]. A series of boronate affinity materials has been reported for bacteria analysis [[17], [18], [19]]. To increase the ...