This paper conducted preliminary investigation on natural genetic transformation of Bacillus subtilis on agar plates. The results showed that, under same conditions, the natural transformation of the strain on agar plate was much more effcient than that of standard liquid method, and the transformation...
subtilis cells. Fifty late L4/young-adult-stage N2 worms were fed either on wild-type spores of domesticated JH642 (red) or undomesticated NCIB3610 (blue) B. subtilis on NGM agar plates, and survival was monitored. (b) Thermotolerance of C. elegans fed on B. subtilis. Thirty late L4/...
subtilis (natto) cells on LB agar plate. Cells (5-μL of overnight preculture grown in liquid LB media) were inoculated on the paper disk placed to the center of LB agar plates (0.8 % [w/v] agar, diameter = 90 mm) and incubate for 2 days at 37°C. Overview of colony ...
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Bacillus subtilis is a model gram-positive bacterium, commonly used to explore questions across bacterial cell biology and for industrial uses. To enable greater understanding and control of proteins in B. subtilis, here we report broad and efficient gen
subtilis from MYP agar plates confirmed the presence of strains L10 and G1 in the GIT of the treated shrimp. On the other hand, the considerable reduction of Vibrio spp. populations in the GIT of shrimp fed BM5 and BM8 diets (Table 3) clearly showed the successful competitive exclusion ...
Bacillus subtilis was grown in C minimal medium supplemented with glucose, succinate, glutamate and auxotrophic requirements (at 50 mg l21) (Commichau et al., 2007). SP (sporulation) and MSgg plates were prepared by the addition of 17 g Bacto agar l21 (Difco) to SP (8 g nutrient broth,...
subtilis and other bacteria. The influence of combing riboswitch with other constitutive promoters on protein expression has not been well characterized until now. To expand the toolbox for conditional gene expression in B. subtilis, a strong constitutive promoter, P43, was placed upstream of the ...
Meanwhile, Bacillus subtilis strain 168 is a standard bacteria strain, so we did not perform additional experiments to identify its strain. Bacteria culture To culture Bacillus amyloiquefaciens for fibril extraction, a frozen stock was streaked onto an Luria–Bertani (LB) agar plate and incubated ...
subtilis, a single colony was first inoculated in LB medium and grown at 37 °C aerobically until reaching an absorbance of 1.0 at 600 nm. Then, 100 μl of the bacteria culture was spread on 2 × yeast extract tryptone agar plates (16 g/L tryptone, 10 g/L yeast extract, 5 g/L ...