In addition, that the LB culture, which usually did not supprt the strain acquire competency, could undergo transformation as soon as it being spread on agar plate, suggested that the natural genetic transformation of Bacillus subtilis on solid plates may be different form the standard liquid ...
Bacillus subtilisis a model gram-positive bacterium, commonly used to explore questions across bacterial cell biology and for industrial uses. To enable greater understanding and control of proteins inB. subtilis, here we report broad and efficient genetic code expansion inB. subtilisby incorporating ...
subtilis (natto) cells on LB agar plate. Cells (5-μL of overnight preculture grown in liquid LB media) were inoculated on the paper disk placed to the center of LB agar plates (0.8 % [w/v] agar, diameter = 90 mm) and incubate for 2 days at 37°C. Overview of colony ...
subtilis subsp. spizizenii based on DNA reassociation studies31 and sbo gene analysis32. The W23 and 168 group strains are identical for most phenotypic characteristics. However, cell wall chemistry of the W23 strains and 168 strains were different; the cell wall of the former contained ribitol ...
Bacillus subtilis strain NCD-2 is strongly antagonistic toward phytopathogenic fungi, and functions as an excellent biocontrol agent for cotton soil-borne diseases. The aims of this study were to characterize the main active antifungal compound from strain NCD-2 and clarify its role in suppressing ...
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Bacillus subtilis, a low G + C%-containing Gram-positive soil bacterium, has been commonly used as a host strain for producing numerous industrial proteins and fine chemicals because of several characteristics, including its status as generally recognized as safe (GRAS), its high cell density, it...
JY14, the number of cells was measured using the standard pour plate method. After lyophilization, the samples were suspended in 3 mL of MB and diluted serially 10-fold diluted in distilled water. After spreading the samples on MB agar plates, the plates were incubated at 30 ℃ for 24 h...
subtilis cells. Fifty late L4/young-adult-stage N2 worms were fed either on wild-type spores of domesticated JH642 (red) or undomesticated NCIB3610 (blue) B. subtilis on NGM agar plates, and survival was monitored. (b) Thermotolerance of C. elegans fed on B. subtilis. Thirty late L4/...
Therefore, the current study was conducted to assess and compare the effects of Bacillus subtilis with growth promoting antibioticon growth performance, nutrient digestibilities, intestinal microbiota, blood characteristics and organ weight changes in broilers after intramuscular inoculation with E. coli. ...