Standardized and well-characterized genetic building blocks allow the convenient assembly of novel genetic modules and devices, ensuring reusability of parts and reproducibility of experiments. In the firstBacillus subtilis-specific toolbox using the BioBrick standard, we presented integrative vectors, promot...
thereplication part, and thecargowhich includes an antibiotic marker for selection inBacillusand a MCS. Therefore, all vectors and sequences offered through theBacillusGenetic Stock Center (BGSC) and the
BGSC, Bacillus genetic stock center; Kanr, kanamycin resistance; MLSr, macrolide-lincosamin-streptogramin resistance. Overproduction and Purification of Recombinant Proteins Expression of dhb gene fragments was carried out under aerobic conditions in 2× yeast tryptone medium supplemented with 10 mm MgCl2...
subtilis strain N10 is more closely related to the B. subtilis reference strain W23 than to 168. The strain, N10, has been deposited in the Bacillus Genetic Stock Center (BGSC) and assigned the accession number 3A17.doi:10.1111/j.1574-6968.2001.tb10551.xEhab...
0 文档热度: 文档分类: 论文--毕业论文 文档标签: 菌一帮助枯草杆菌反馈意见 系统标签: bacillussubtilis枯草芽孢杆菌bgscvolumestrains Bacillus Genetic Stock Center Catalog of Strains, Seventh Edition, Volume 1: Bacillus subtilis 168 © 2000 Permission is given to copy this material or portions of it ...
BGSC: Bacillus Genetic Stock Center, Ohio State University TLC: thin-layer chromatography HMF: 5-hydromethylfurfural OD: optical density xylA: xylose isomerase gene xylB: xylulose kinase gene xylR: regulator gene of xylose metabolism operon EGTA: ethylene glycol bis(2-aminoethyl) tetraacet...
1. A Bacillus strain identified by Bacillus Genetic Stock Center Accession Number PW1 (BGSC PW1). 2. A method for controlling mosquitoes comprising contacting a mosquito larva with a toxic amount of the Bacillus strain of claim 1, or its crystals, crystal proteins, protoxins, or toxins. 3...
The insertionalsda::ermandlexA::ermdeletion mutants (BKE25690 and BKE17850, respectively) were purchased from theBacillusGenetic Stock Center (BGSC,http://www.bgsc.org) and introduced into NCIB 3610 via SPP1 phage-mediated transduction8,36to generate YCN025 and YCN020, respectively. To allow...
The strain HD521 belongs to the Indiana subspecies (Indiana) and was obtained from Bacillus Genetic Stock Center (BGSC). Strain HS18-1 was isolated from the Sichuan Basin of China and was stored in our laboratory. Genomic extract reagent (QIAGEN Genomic-tip 500/G) was purchased from QIAGENgs...
01-1 Kmr; PA+Example 1 below *alsA and ccpA genes are allelic (Henkin et al., Molecular Microbiology, (1991) 5, 575-584) .dagger.Bacillus Genetic Stock Center, The Ohio State University, Columbus, Ohio, USA. .dagger-dbl.Protective Antigen ...