subtilis46, plasmids for C-terminal fusions to lacZ or other reporter genes offered by the Bacillus Genetic Stock Center are not properly insulated. It is possible that incorporation of a single terminator could greatly improve the limit of detection of future assays based on these plasmids. The ...
and produces small amounts of vitamins B12and K. The bacterium, which is also found in soil and water, is widely used in laboratory research and is said to be the most thoroughly studied life form. Ingenetic engineeringit is the microorganism preferred for use as a host for the gene-splic...
thereplication part, and thecargowhich includes an antibiotic marker for selection inBacillusand a MCS. Therefore, all vectors and sequences offered through theBacillusGenetic Stock Center (BGSC) and the
Bacillus Genetic Stock Center, Department of Microbiology, The Ohio State University, 484 W. 12th Avenue, Columbus, OH 43210, U.S.A.Donald H.DeanBacillus Genetic Stock Center, Department of Microbiology, The Ohio State University, 484 W. 12th Avenue, Columbus, OH 43210, U.S.A....
<br/>Bacillus Genetic Stock Center Catalog of Strains, Seventh Edition, <br/>Volume 1: Bacillus subtilis 168 <br/> <br/> <br/>© 2000 <br/>Permission is given to copy thi..
Rajasekar Vinayagam Austin Publishing Group Materials and Methods Microorganism and chemicals Bacillus subtilis 1A752 strain was procured from Bacillus Genetic Stock Center, USA as a gift sample for present work. For routine work the bacteria was maintained on nutrient agar slants. The slants wer...
1. A Bacillus strain identified by Bacillus Genetic Stock Center Accession Number PW1 (BGSC PW1). 2. A method for controlling mosquitoes comprising contacting a mosquito larva with a toxic amount of the Bacillus strain of claim 1, or its crystals, crystal proteins, protoxins, or toxins. 3...
subtilis wild-type 168 (W168), was obtained from the Microbe Division at the RIKEN BioResource Research Center, Japan. We used the CRISPR-Cas9 system for gene knockout in B. subtilis [30]. Plasmid pJOE8999 was obtained from the Bacillus Genetic Stock Center (Ohio State University, Columbus,...
including 33 cultivars for stiff-stock (SS), 143 for non-stiff-stock (NSS), 232 for tropical and semi-tropical (TST), and the left 119 cultivars for mixed, encompassing adequate genetic diversity and covering most variations for the traits of interest (Supplementary Table 1). We hypothesized...
subtilis strain 1A40 (Bacillus Genetic Stock Center, Columbus, OH) was PCR amplified as an EcoR1-BamH1 fragment. The product was cloned into pDG1661 at a site directly upstream of the lacZ reporter gene. Mutants were created within the engineered pDG1661 by using the appropriate primers and ...