Cytotoxic T Lymphocyte-associated antigen 4 (CTLA-4) is an important costimultory receptor expressed on activated T cells. CTLA-4 blockade using a monoclonal antibody (mAb) in conjunction with tumor vaccines has improved tumor responses in animal models and enhanced numerous models of T...
A tumor model was established in C57BL/6J mice via subcutaneous injection of 3×105B16-OVA tumor cells. The effect of alginate/OVA peptide on cell viability was analyzed by use of the CCK-8 assay kit. Activation of macrophages was examined by checking cell surface makers CD40 and CD86 by ...
表达单纯疱疹病毒受体HVEM的黑色素瘤B16-ova-HVEM细胞系的构建及成瘤
28.p.c.rosato,s.wijeyesinghe,j.m.stolley,c.e.nelson,r.l.davis,l.s.manlove,c.a.pennell,b.r.blazar,c.c.chen,m.a.geller,v.vezys,d.masopust,virus-specific memory t cells populate tumors and can be repurposed for tumor immunotherapy,nat.commun.10,567(2019).[0268]33.s.giguè...
Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell suspension to new culture vessels. 6. Incubate cultures at 37°C. ...
Although the mechanisms by which dasatinib alters the metastatic behavior of B16-OVA cells in vivo remain to be determined, we hypothesize that dasatinib acts via multiple tumor-extrinsic processes that include immune function and neoangiogenesis.doi:10.4161/cbt.10.7.12926...
Immunization with OVA and fucoidan protects mice from challenge with B16-OVA tumor cells.JunO, JinWei, ZhangJiangYuan, DuKaWing, WongTatsuya, OdaQing, Yu
B16-OVA Cell LineCat No.: NTCC501219B16-OVA is an OVA-transfected clone derived from the murine melanoma cell line B16. The tumor cell line was cultured in vitroin CM in the presence of geniticin (2 mg/mL) and hygromicin B (60 μg/mL).Culture Medium:DMEM