Callus proliferation and metabolism were assessed through biometric measures, non-structural soluble carbohydrates quantification, osmotic potential determination, as well as by quantification of mRNA accumulation of B-type cyclin genes. Calli were cultured on proliferation medium without sucrose or ...
Abstract Cyclin-dependent kinases (CDKs) activate the firing of replication origins during the S phase of the cell cycle. They also block re-initiation of DNA replication within a single cell cycle, by preventing the assembly of prereplicative complexes at origins. We show here that, in budding...
In Saccharomyces cerevisiae, Fkh2 both activates and represses transcription of CLB2, encoding a B-type cyclin. CLB2 is expressed during G(2)/M phase and repressed during G(1). Fkh2 recruits the coactivator Ndd1, an interaction which is promoted by Clb2/Cdk1-dependent phosphorylation of ...
We placed the expression of this mitotic cyclin under the control of tetracyclin-regulatable promoter, which enabled us to express it ectopically in other phases of the cycle. The expressed CycB2;2, protein could be found in S- and G2-phase cells, where it became located into the nucleus....
Article: Caenorhabditis elegans cyclin A- and B-type genes: a cyclin A multigene family, an ancestral cyclin B3 and differential germline expressio
肝右叶肿物穿刺活检( 图3 ),免疫组化结果,CD20(+),Bcl-2(+),Bcl-6(+),C-myc(30%阳性),Pax-5(+),MUM1(+),Ki67(阳性细胞数70%),AE1/AE3、CD3、CD38、CD10、CD21、CD30、CyclinD1均阴性,原位杂交结果:EBER (-),倾向非霍奇金弥漫大B细胞淋巴瘤。 展开阅读 图3 肝脏穿刺病理结果 可见异型...
免疫组织化学示:大细胞CD30、CD15、BOB1、EMA、CK均阴性,CD20、PAX5、CD79a、Ki-67、OCT2、LCA均阳性;T细胞CD4、CD8、CD3、CD5均阳性,SOX11、CYCLIND1均阴性,CD57、bcl-2、CD10、MUM1部分阳性,bcl-6少量阳性,EBER阴性。诊断为NLPHL,Ⅲ A期,国际预后评分(IPS)1分,予ABVD方案(吡柔比星+博来霉素+长春地...
Two new B-type cyclin genes from Saccharomyces cerevisiae, called CLB5 and CLB6, are located in a tail to tail arrangement adjacent to the G2/M phase promo... Kühne,Linder - 《Embo Journal》 被引量: 167发表: 1993年 Use of B-type natriuretic peptide in the management of hypoxaemic ...
Cell-cycle-regulated transcription of A- and B-type plant cyclin genes in synchronous cultures Synchronously dividing cell cultures of Catharanthus roseus were used to isolate cDNAs for two mitotic cyclins, named CYS and CYM. The deduced protein sequ... Ito,MarieClaire,Sakabe,... - 《Plant Jour...
组织学检查提示恶性淋巴样细胞弥漫性侵润,非典型淋巴样细胞浸润,如图4。免疫组化染色显示CD20(+),BCL-6(>30%),MUM-1(>30%)呈阳性,对CD3,CD10,Cyclin-D1,Tdt,CD30曾呈阴性,ALK,Ki67增殖指数>80%,更加证实了弥漫性大B细胞非霍奇金淋巴瘤。 图4 术后大体标本...