Intra-Assay: CV<10% Inter-Assay: CV<12% Stability of the ELISA Kit for Hexosaminidase B Beta (HEXb) The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize ...
Assay of Mutant Proteins Enzyme activity was determined with MU-GlcNAc and MU-GlcNAc-6-SO4 as described previously (9). Protein concentrations were measured according to the method of Bradford (15) using bovine serum albumin as a standard. [3H]GM2 ganglioside, labeled in the GalNAc moiety, wa...
The serum Hex assay by the heat-inactivation method performed at that time showed about 20% of residual Hex A activity. General convulsions first occurred at the age of 18 months and have since increased despite anticonvulsant therapy. The rectal biopsy, performed with the informed consent of ...
The release of tumor necrosis factor α was analysed by using enzyme-linked-immunosorbent-assay, and the results indicated that neither lipopolysaccharide, ionomycin, nor 5'-(-ethylcarboxamido) adenosine had any effect on this process. 展开
Specificity of Human Glucosylceramide -Glucosidase Towards Structurally Modified Glucosylceramides in a Liposomal Assay-System Glycosphingolipids are the most abundant glycolipids in the plasma membrane of animal tissues. Their degradation occurs in lysosomes, where specific hydrolytic enzymes cut off the ...
Expression and Assay of Humanβ-1,2-N-acetylglucosaminyltransferase Ⅰin Pichia Pastoris; β-1,2-N-乙酰葡萄糖胺转移酶Ⅰ(beta-1,2-N-acetylglucosaminyltransferase Ⅰ,GnTⅠ)是哺乳动物细胞糖蛋白N-糖链加工的关键酶之一,催化糖链由甘露糖型向杂合型转变。
PURPOSE:The titled measuring reagent, containing a specific compound as a substrate, and capable of carrying out rate assay accurately and easily in a short time. CONSTITUTION:A reagent obtained by incorporating a compound expressed by the formula [A is N-acetylglucosamine or N-acetylgalactosamine ...