Cell Biolabs’ Beta Hexosamindase Activity Assay Kit is a simple fluorometric assay that measures beta hexosaminidase activity in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, beta hexosaminidase positive control and unknown samp...
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A continuous fluorimetric assay for glycosidase activity: human N-acetyl-beta-D-hexosaminidaseThe fluorescence intensity of 4-methylumbelliferone (λ excitation = 320 nm, λ emission = 450 nm) is approximately 120-fold greater than those of 4-methylumbelliferyl glycosides over a pH range from 3 to...
Inter-Assay: CV<12% Stability of the ELISA Kit for Hexosaminidase B Beta (HEXb) The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the ...
PURPOSE:The titled measuring reagent, containing a specific compound as a substrate, and capable of carrying out rate assay accurately and easily in a short time. CONSTITUTION:A reagent obtained by incorporating a compound expressed by the formula [A is N-acetylglucosamine or N-acetylgalactosamine ...
ml.Recovery: The recovery of this analyte spiked is evaluated, the range is 89%-112%, the average is 99%.Specificity: No significant cross-reactivity or interference between this analyte and analogues is observed.Reproducibility: Both Intra-assay CV (%) and Inter-assay CV (%) is less than...
The enzymatic activity of HexA in the plasma of the patient was assessed using a fluorescent substrate assay. The HexA 伪-subunit (HexA) concentration was determined using ELISA. It was shown that both UCBCT and curcumin administration led to a change in the patient's cytokin...
PURPOSE:The titled measuring reagent, containing a specific compound as a substrate, and capable of carrying out rate assay accurately and easily in a short time. CONSTITUTION:A reagent obtained by incorporating a compound expressed by the formula [A is N-acetylglucosamine or N-acetylgalactosamine ...
Upon enzyme hydrolysis, 4-MU is released which is highly fluorescent, the intensity of which is used to measure enzyme activity.Using the above in vitro method, we identified two families in the Lebanese population with Gaucher disease. The first family was homozygous for L37/Vand-the second ...