Ig gene rearrangementJH locustargeted mutationB lymphocyte differentiation is characterized by an ordered series of Ig gene assembly and expression events. In the majority of normal B cells, assembly and expression of Ig heavy (H) chain genes precedes that of light (L) chain genes. To determine...
We describe the use of the Southern blot hybridization technique to diagnose B-cell lymphoma by detecting clonal immunoglobulin gene rearrangements in lymph node and other biopsy tissues. DNA was isolated from a wide variety of neoplastic and non-neoplastic specimens and analyzed for the presence of...
Immunoglobulin gene rearrangement in B cell deficient mice generated by targeted deletion of the JH locus. Int. Immunol. 5, 647–656 (1993). Article CAS PubMed Google Scholar Filali, M. et al. Cognitive and non-cognitive behaviors in the triple transgenic mouse model of Alzheimer’s ...
of the B cells showed one V(D)J recombination pattern. Intriguingly, however, two or more VHDJHor VLJLrecombination patterns of IgH chain or IgL chain were also observed in hundreds to thousands of single B cells. Moreover, each Ig class showed unique VHDJHrecombination pattern in a single ...
The vertebrate B-cell repertoire is capable of generating up to 10(9) different antibody molecules using relatively few germline immunoglobulin (Ig) gene segments. To generate diversity, humans and mice depend on combinatorial and junctional variations that occur during the gene rearrangement events tha...
The earliest stage is distinguished by accessibility of the immunoglobulin (Ig) heavy chain locus, indicating chromatin changes preparatory to heavy chain rearrangement. Upon activation of the recombinase complex, first a diversity (D) region segment rearranges to one of four joining (J) segments (...
The B cell receptor (BCR) is encoded by the same rearranged gene as encodes the immunoglobulin (Ig) that its later developmental offspring will secrete. This gene rearrangement is crucial to the diversity of the B cell repertoire—enabling a large number of different specificities of Ig in order...
These mechanisms may operate alone or in combination to influence V gene rearrangement and populations of immature B cells. Although constraints on the pool of rearranged V genes may seem disadvantageous to the immune system, the mechanisms that generate the CDR3s of heavy and light chains ensure...
Immunoglobulin and T-cell receptor gene rearrangement and expression in human lymphoid leukemia cells at different stages of maturation The use of probes to genes (IG and TCRB) encoding immunoglobulins (IG) and the 尾 chain of the T-cell antigen receptor (TCRB), respectively, have become a ....
1993. RAG-1 and RAG-2 are not sufficient to direct all phases of immunoglobulin gene rearrangement in pre-B-cell lines. Mol. Cell. Biol. 13: 3890-3899.Wang LC, Rosenberg N: RAG-1 and RAG-2 are not sufficient to direct all... LC Wang,N Rosenberg - 《Molecular & Cellular Biology》...