SOX2 (A0561) and β-actin (AC026) were purchased from Abclonal. ITPR2 (DF13336) was obtained from Affinity. E-cadherin (3195), N-cadherin (13116), Vimentin (5741), NF-κB (8242), Phospho-NF-κB (Ser536) (3033), Phospho-IκBα (Ser32) (2859), Snail1 (3879), and Snail 2 (...
; Flag-tag (390002), HA-tag (301113) were obtained from ZENBIO; GST-tag (T0007), Flag-tag (T0053, 1:100 for IP) were purchased from Affinity; GRP78 (66574–1-Ig, 1:50 for IP) was purchased from Proteintech, HA-tag (AE008, 1:100 for IP) was purchased from Abclonal....
The primary antibodies used in immunoblotting are as follows: β-actin (1:2000, Proteintech, 20536-1-AP), hnRNP F (1:1000, Thermo, PA522341), hnRNPA1 (1:1000, Cell Signaling Technology, #8443), hnRNPA2B1 (1:1000, ABclonal, A1162), p-IKKα/IKKβ (1:1000, Cell Signaling ...
RARB (1:1000, ABclonal, Wuhan, China) β-actin (1:1000, ABclonal, Wuhan, China) E-Cadherin (1:1000, proteintech, Chicago, USA) N-Cadherin (1:1000, ABclonal, Wuhan, China) Vimentin (1:1000, ABclonal, Wuhan, China) HRP Goat Anti-Rabbit IgG(H + L)(1:20000, ABclonal, Wuhan,...
Antibodies against Alix (A2215), TSG101 (A2216), and VDAC1 (A0810) were purchased from ABclonal. Peroxidase-labeled β-actin antibody (A3854) and Flag antibody (F3165) were purchased from Sigma. nSMase2 (sc-166637) antibody was purchased from Santa Cruz Biotechnology. HA antibody (M20003...
After CAFs are activated, phenotype transformation and the expression of their markers, such as alpha smooth muscle actin (α-SMA) and fibroblast activation protein (FAP), are upregulated. Different subtypes of CAFs have been shown to be related to negative outcomes in patients with gastric ...
tissues was obtained by centrifuging at 4500 rpm, 4 °C, for 15 min. The protein concentration in the supernatant was determined using a BCA protein quantification kit, and the levels of iNOS (M1 marker) and Arg-1 (M2 marker) were measured using specific ELISA detection kits (ABclonal, ...
(3033, Cell Signaling Technology), LaminB1 (66095-1-Ig, Proteintech), ubiquitin (10201-2-AP, 1:1000, Proteintech), CCL20 (A1756, Abclonal), HA (3724, Cell Signaling Technology), Flag (AF5051, Beyotime), Ki-67 (27309-1-Ig, Proteintech), β-actin (M1210-2, HuaBio), and GAPDH (...
Progesterone Receptor Rabbit monoclonal A22061 Company ABclonal, Inc. CD36 Rabbit monoclonal A5792 Company ABclonal, Inc. PPARγ Rabbit monoclonal #8660 Cell signaling technology ACC Rabbit monoclonal #3676 Cell signaling technology Phosphor-ACC Rabbit monoclonal #3661 Cell signaling technology SREBP-1 Mo...
ACC1 (1:1000) ABclonal, China A15606 RRID:AB_2763012 ApoE (1:1000) Santa Cruz Biotechnology, USA sc-98574 RRID:AB_2058119 HRP-conjugated β-actin (1:3000) ABclonal, China AC028 RRID:AB_2769861 BRCA1 (1:1000) ABclonal, China A11034 RRID:AB_2758380 BARD1 (1:1000) ABclonal, China A...