Antibodies against phospho-IκBα, phospho-IRF3, NEDD8, and UBA3 were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against IκBα and actin and NF-κB inhibitor JSH-23 (dissolved in dimethylsulphoxide, DMSO) were purchased from Santa Cruz (Santa Cruz, CA, USA)....
(anti-rabbit) was purchased from ABclonal (Waltham, MA, USA). The β-actin-Cat. #A5441 (anti-mouse) endogenous control was purchased from Sigma, St. Louis, MO, USA. The secondary HRP-linked anti-mouse-Cat. #7076 and anti-rabbit-Cat. #7074 were purchased from Cell Signaling Technology...
The qPCR was conducted using the Rotor-Gene RG3000 instrument (Montreal Biotech Inc., Dorval, QC, Canada) with the 2× Universal Sybr Green Fast qPCR Mix (AbClonal) following the manufacturer’s protocol. Data are represented as fold change to the control or LPS-treated group after ...
(anti-rabbit) was purchased from ABclonal (Waltham, MA, USA). The β-actin-Cat. #A5441 (anti-mouse) endogenous control was purchased from Sigma, St. Louis, MO, USA. The secondary HRP-linked anti-mouse-Cat. #7076 and anti-rabbit-Cat. #7074 were purchased from Cell Signaling Technology...
EXOC6 and EXOC6B (EXOC6/6B) components of the exocyst complex are involved in the secretory granule docking. Recently, EXOC6/6B were anticipated as a molecular link between dysfunctional pancreatic islets and ciliated lung epithelium, making diabetic pat