The 20 µl diluted crypts were mixed with 20 µl matrix gel (356231, Corning, NY, USA), and the mixture was seeded into a 48-well plate. After fixation in an incubator at 37 °C for 15 min, 200 µl of culture medium (IntestiCult™ Organoid Growth Medium, STEMCELL Technologies...
Yes-associated protein 1 (YAP1) regulates the survival, proliferation, and stemness of cells, and contributes to the development of metabolic dysfunction associated fatty liver disease (MAFLD). However, the regulatory role of intestinal YAP1 in MAFLD sti
Department of Information and Computer Science, University of California, Irvine, CA (1994) Google Scholar [29] J.N. Narvaez, B.K. Lavine, P.C. Jurs Structure-activity studies of musk odorants using pattern recognition: bicyclo- and tricyclo-benzenoids Chem. Senses, 11 (1986), pp. 145-15...
The Cancer Genome Atlas (TCGA) for lung SCC revealed frequent mutations in receptor tyrosine kinases (RTKs), AKT and PI3K, which converge to activate the mechanistic target of rapamycin (mTOR) signaling pathway (Ding et al., 2008, Cancer Genome Atlas Research Network, 2012). The mTOR pathway...
DNA was extracted and purified from hiPSCs using the Quick-DNA Miniprep kit (#D3024, Zymo Research Corp. Irvine, CA, USA). 300 ng of DNA was used to perform karyotyping using hPSC Genetic Analysis Kit (#07550, STEMCELL Technologies Inc., Vancouver, Canada) according to the manufacturer’s...
1 ARTICLE NATURE COMMUNICATIONS | DOI: 10.1038/ncomms3174 The reprogramming of differentiated cells into induced pluripotent stem cells (iPSCs) by introduction of four defined transcription factors (Oct4, Sox2, Klf4 and Myc) has rapidly become an intensively investigated area in stem cell research ...
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An apparatus is fabricated with a plurality of semiconductor-device substrates and/or MEMS substrates with micromachined sensors, circuits, transducers, and/or MEMS devices fabricated on the plurality
2. Materials and methods 3. Results 4. Discussion Acknowledgements Appendix A. Supplementary material Research Data ReferencesShow full outline Cited by (12) Figures (11) Show 5 more figures Extras (11) Download all Movie S1 Movie S2 Movie S3 Movie S4 Movie S5 Movie S6Show all extrasDevelo...
Quantitative PCR reactions were run on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Irvine, CA) using PowerUp™ SYBR™ Green Master Mix (Thermo Fisher Scientific). Primers were designed using Primer Bank and purchased from Integrated DNA Technologies. qRT-PCR analysis was ...