Target gene mRNA was amplified with SYBR® Premix Ex Taq™ II kit (Code No. RR820A, Takara Bio) and measured by CFX96™ Real-time System (Bio-Rad, Irvine, CA, USA). Each single reaction system (15 µL) consisted of 7.50 µL 2× KAPA SYBR Fast qPCR Master Mix Universal,...
Nature Communications volume 15, Article number: 4995 (2024) Cite this article 5319 Accesses 1 Altmetric Metrics details Abstract RNF214 is an understudied ubiquitin ligase with little knowledge of its biological functions or protein substrates. Here we show that the TEAD transcription factors in th...
About RFaxis, Inc. Incorporated in January 2008, RFaxis, Inc. is an Irvine, CA, based company specializing in the design and development of RF semiconductors. With its patented technologies, the company leads the way in next-generation wireless solutions designed for the multibillion dollar Bluet...
Briefly, cells were harvested following a 1-h treatment with 0.01 mg ml À 1 Colcemid (Irvine Scientific, Santa Ana, CA) and ethidium bromide 1 mg ml À 1, hypotonic KCl (0.075 M) treatment for 30 min, followed by fixation and washes in 3:1 acetone-free methanol: glacial acetic ...
Blood pressure and central hemodynamics were assessed via a Nexfin device (Edwards Lifesciences, Irvine, CA, USA) that utilizes the volume-clamp method to measure blood pressure and heart rate, while cardiac output was estimated using the CO-trek algorithm [65]. Heart rate variability, a marker...
Launched in March 2009, RFaxis, Inc. is an Irvine, CA, based company specializing in the design and development of RF semiconductors. With its patented technologies, the company leads the way in next-generation wireless solutions designed for the multibillion dollar WLAN 802.11b/g/a/n/ac, MI...
1. Department of Psychiatry, Kaiser Permanente Medical Center, 975 Sereno Drive, 94589-2485, Vallejo, CA 2. Department of Psychiatry and Human Behavior, University of California, Irvine Medical Center, 101 City Drive South, 92668, Orange, California Continue...
Genomic DNA was isolated using Quick-gDNA MicroPrep (Zymo, Irvine, CA, USA; D3021) according to the manufacturer’s instructions. Quantitative methylation-specific PCR (QMSP) Genomic DNA was extracted from ARK2 and MCF-7 cells in one well of six-well plates using Quick-gDNA MicroPrep. For ...
The evolutionarily conserved Hippo pathway is a regulator that controls organ size, cell growth and tissue homeostasis. Upstream signals of the Hippo pathway have been widely studied, but how microenvironmental factors coordinately regulate this pathway
RUTAN & TUCKER, LLP (Irvine, CALIFORNIA, US) Claims: 1.A machine-implemented method for placement of one or more rows of vertical axis wind turbines in a plot of land using an automatic wind turbine placement device, comprising:placing and arranging the rows of two or more vertical axis wi...