最后研究人员评估了AvrPtoB磷酸化对植物细菌生长的影响。生长曲线分析显示,Pst(∆avrPtoB)和Pst(avrPtoBS335D)表现出相似的毒性。与PTI响应一致,Pst(avrPtoBS335D)感染产生的细菌滴度比Pst、Pst(avrPtoBWT)或Pst(avrPtoBS335A)感染产生的细菌滴度低得多。这些结果表明,在S335位点AvrPtoB的磷酸化减弱了其毒性...
AvrPtoBPto 基因酵母菌雙雜交法與病原性測試 Pseudomonas syringaetype III secretion system (T3SSeffector proteinsAvrPtoBPto locusPseudomonas syringae, a Gram-negative rod-shaped bacterium, can infect a wide array of host plants and cause different disease symptoms. Due to its broad host range and ...
AvrPtoB is a type III effector protein from Pseudomonas syringae pv. tomato that physically interacts with the tomato Pto kinase and, depending on the host genotype, either elicits or suppresses programmed cell death associated with plant immunity. We reported previously that avrPtoB-related sequence...
番茄细菌性斑点病是影响番茄产量和品质的重要病害,Pseudomonassyringaepv.tomato(Pst)为其病原菌,Pst与番茄的互作系统是研究植物抗感病机理的典型模式系统.Pst存在两种无毒基因:AvrPto和AvrPtoB,它们编码的蛋白质均能与番茄抗病基因Pto编码的Ser-Thr蛋白激酶互作,符合Flor"基因对基因"学说.AvrPto和AvrPtoB在表达Pto的抗...
AvrPtoB ubiquitinates the CERK1 kinase domain in vitro and targets CERK1 for degradation in vivo. We show that CERK1 is a determinant of bacterial immunity, but its contribution is overcome by bacteria expressing AvrPtoB. Our results reveal a new pathway for plant immunity against bacteria ...
摘要:【目的】利用酵母双杂交系统在感病番茄中蔬四号中筛选分别与AvrPto、AvrPtoB互作的蛋白,研究 AvrPto、AvrPtoB作为毒性因子在感病番茄中的作用通路。【方法】提取感病番茄中蔬四号的总RNA,合成中蔬四 号cDNA文库,分别构建AvrPto和AvrPtoB的诱饵载体,从中蔬四号cDNA文库中筛选分别与AvrPto、AvrPtoB互 ...
tomato DC3000 Type IIISecretion Effector Polymutants Reveal an Interplaybetween HopAD1 and AvrPtoBGraphical AbstractHighlightsd An effectorless Pseudomonas syringae mutant wasconstructed to study effector interplayd The effector HopAD1 is suff i cient to elicit cell death inNicotiana benthamianad An ...
Structure of the BAK1 kinase complexed with the pathogen effector AvrPtoB was solved AvrPtoB has duplicated kinase-binding domains in its N-terminal regio... C Wei,K Munkvold,H Gao,... - 《Cell Host & Microbe》 被引量: 252发表: 2011年 Deletions in the Repertoire of Pseudomonas syringae...
通过荧光素酶互补,体内免疫共沉淀等实验,我们证实了CYP707A1和CYP707A2可以与效应蛋白AvrPtoB互作.体内泛素化实验证明AvrPtoB可以泛素化CYP707A1和CYP707A2并导致其降解,并且该降解途径依赖于26S蛋白酶体.CYP707A编码的是一个8''-ABA-羟化酶,其泛素化降解会增加植物体内ABA的含量,可能是导致植物对入侵致病菌...
(2005) Avrptob: a bacterial type III effector that both elicits and suppresses programmed cell death asso- ciated with plant immunity. FEMS Microbiol. Lett. 245, 1-8.Abramovitch RB,Martin GB.AvrPtoB:a bacterial type IIIeffector that both elicits and suppresses programmed celldeath associated ...