To break the avidin-biotin interaction, 8 M guanidine• HCl at pH 1.5 or boiling in SDS-PAGE sample buffer is required. These elution methods may result in denaturation of the biotinylated protein and cause irreversible damage to the support. In addition, avidin orI Iminobiotin...
Because carbohydrate is found selectively on the Fc portion of antibodies, biotin labeling is restricted from occurring near the antigen-binding site. This site-specific labeling method provides an antibody conjugate with the highest possible specific activity that is particularly important when antibody ...
是一种蛋白质,具有非常高的亲和性,可以与Biotin进行特异性的结合。 来源于不同的生物体,如鸡的蛋黄、链霉菌属等,其中Avidin是常见的形式,但也有Striptavidin(来源于链霉菌属)和Neutravidin(人为改造的去糖基化的亲和素)等其他类型。 Avidin是一个四聚体,由四个相同的亚基组成,结构上具有四个biotin-binding的结构...
a co-factor that plays a role in multiple eukaryotic biological processes. Avidin and other biotin-binding proteins, including Streptavidin and NeutrAvidin protein, have the ability to bind up to four biotin molecules, as shown in the di...
产品名称Avidin, Aladdin™, Oregon Green™ 488 conjugate 别名亲和素、Aladdin™、Oregon Green™ 488 结合物 产品介绍 Product introduction: OurOregon Green™ 488 conjugate of NeutrAvidin™ biotin-binding protein- a form of avidin that has been processed to remove carbohydrate and to lower its...
Avidin–Biotin Complex (ABC) staining method Reporter intensity is a function of the localized enzyme activity, and improved sensitivity can be achieved by increasing the number of enzyme molecules bound to the target antigen. The multiple bi...
Desc:Avidin (Biotin Binding Protein) is a strongly basic glycoprotein synthesized in the hen oviduct and deposited in the albumen fraction of eggs at a ratio of 0.05% of the total protein content. It binds the water-soluble vitamin H (d-biotin) very tightly and specifically with a binding ...
The specific activity for biotin binding is approximately 14 µg/mg of protein, which is near the theoretical maximum activity. 生物活性 Binding Biotin 偶联 HRP 来源 天然 预测分子量 60kDa AI解读 图片 产品规格参数 应用 质检证书(CoA,COO,BSE/TSE 和分析图谱) 相关文档 溶液计算器...
The irreversible nature of the binding also allows for the procedure to be undertaken with the most stringent of conditions.doi:10.1385/1-59259-213-9:203Gary L. BratthauerHumana PressMethods in Molecular BiologyBratthauer, G.L. 1999. The Avidin-Biotin Complex (ABC) Method and other Avidin-...
Biotin binding and elution with Monomeric Avidin Agarose 技术参数 实验方法和说明书下载 SepTube™密度梯度离心管-50(20根/包) 简单:添加血液样本简单; 快速:离心仅需10分钟(brake on); 操作简捷:仅需倾倒收集细胞,无需小心吸取; 一致性:降低误差,减少不同操作人员间的操作差异。