The Labeled Streptavidin–Biotin (LSAB) staining method employs a streptavidin–enzyme conjugate to detect the bound biotinylated primary antibody on the tissue section and can be used if the avidin–biotin–enzyme complex in ...
The avidin-biotin complex is the strongest known noncovalent interaction (Ka = 1015 M-1) between a protein and ligand. The bond formation between biotin and avidin is very rapid and, once formed, is unaffected by extremes of pH, temperature, organic solvents and other denaturing agents. These...
Avidin–Biotin Complex (ABC) staining method Reporter intensity is a function of the localized enzyme activity, and improved sensitivity can be achieved by increasing the number of enzyme molecules bound to the target antigen. The multiple bi...
产品名称:avidin-biotin-peroxidase complex产地:西安供应商:西安齐岳生物用途:仅供科研纯度:95%+包装:瓶装保存:冷藏相关推荐:生物素-PEG-8-羟基喹啉-5-羧酸生物素接枝紫草素鸦胆子素 A-生物素生物素-5-羰基-8-羟基喹啉FP-生物素_CAS:259270-28-5羌活醇和D-生物素接枝生物素(biotin)连接的精胺(spermine)生物素...
产品名称:avidin-biotin-peroxidase complex 简介: 在实验中,首先将生物素标记的一抗(一般是抗体)与待检组织或膜上的靶分子结合,然后再加入Avidin-Biotin-Peroxidase Complex。由于生物素和鸟蛋白之间的非常强的亲和力,Avidin-Biotin-Peroxidase Complex能够非常牢固地结合在生物素标记的一抗上。接着,加入过氧化物酶底物...
1990. Avidin-biotin complex incor- poration into enzyme-linked immonosorbent assay (ABELISA) for improving the detection of Azospirillurn brasilense Cd. Curr. Microbiol. 20: 91 -94.Levanony, H. y Y. Bashan. 1990. Avidin-biotin complex incorporation into enzyme-linked immunosorbent assay (...
The Avidin-Biotin Complex (ABC) method and other avidin-biotin binding methods. Immunocytochemical Methods and Protocols. (Methods in Molecular Biology 115). In: Javois LC, Ed. Totowa, NJ. Humana Press Inc., 1999; pp. 203-14.The avidin-biotin complex (ABC)method and other avidin-biotin ...
A comparative study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radioimmunoassay ant... A highly sensitive immunoenzymatic technic is presented. The method involves three sequential steps: (1) primary antibody, (2) biotin-labeled ...
The crystal structures of a deglycosylated form of the egg-white glycoprotein avidin and of its complex with biotin have been determined to 2.6 and 3.0 , respectively. The structures reveal the amino acid residues critical for stabilization of the tetrameric assembly and for the exceptionally tight...
avidin-biotin-peroxidase complex method, ABC method 抗生物素蛋白-生物素-过氧化物酶复合物法文献(pubmed) 赞助商链接以下为句子列表:英文: Microcantilever Biosensor on the Biotin-avidin Interaction中文: 微悬臂生物传感器检测生物素-亲和素相互作用