the length of such k-mismatch substrings and we describe kmacs, an efficient implementation of this idea based on generalized enhanced suffix arrays.Results: To evaluate the performance of our approach, we applied it to phylogeny reconstruction using a large number of DNA and protein sequence ...
ANI Average nucleotide identity between the query and reference sequences len_1 The length of the query sequence len_2 The length of the reference sequence ani_alnlen The total length of aligned nucleotides between the query and reference sequences af_1 Alignment fraction of the query sequence ...
Different from structure-based routes, MLAB exploits a strategy that not only extracts local evolutionary information from primary sequences, but also using predicts solvent accessibility. Moreover, the construction about predictors of DNA-protein binding sites wields an ensemble weighted sparse ...
The loops connecting the beta-strands vary in length and structure. It is these loops that give the fold its varied binding capability and the binding sites lie in different parts of the fold. The beta-trefoil proteins have little sequence similarity (sometimes less than 17%) and bind a ...
What occurs during the phase of protein synthesis called transcription? A) mRNA is produced from a DNA template. B) a polypeptide is assembled using the base sequence in mRNA as a guide. C) ribosomes are being assembled at the nucleoli. D) tRNA ...
PolyQ length affects GST-FLAG-HTTexon1 quantification by MSD assay using polyQ targeting detection Ab. (a) Diagram shows antibody epitopes in human HTT protein (NCBI reference sequence: NP_002102.4). Calibration curve performance for GST-FLAG-HTTexon1 protein using MW1 (b) and MAB5492 (c) det...
Telomeres have emerged as crucial cellular elements in aging and various diseases including cancer. To measure the average length of telomere repeats in cells, we describe our protocols that use fluorescent in situ hybridization (FISH) with labeled pepti
How to average data collected from related taxa may be the most basic problem in which the vagaries of phylogenetic relationships come into play. Because of the dependence structure imposed by the phylogenetic tree, it is inappropriate to treat sequences or other data collected from extant species...
have been identified based on protein sequences [42]. Therefore, the scope for functional redundancy in this family is high, and gene knock-outs might not reveal clear phenotypes. In addition, no PMEs have been biochemically characterised in this species, and some may actually be pectin trans-...
e.g. basement membrane rich in a cell adhesion peptide such as one composed of arginine-glycine-aspartic acid (RGD) so that the cells believe they are located on a compatible surface which encourages growth, the cells will grow. RGD is the peptide sequence in cell adhesive proteins such as...