Autophagy-related protein 8 (Atg8) family interacting motif in Atg3 mediates the Atg3-Atg8 interaction and is crucial for the cytoplasm-to-vacuole targeting pathway. J Biol Chem. 2010; 285 :29599–29607.Yamaquchi M,Noda NN,Nakatogawa H. Autophagy-related protein 8(Atg8)family interacting ...
Currently, there is only one Atg8 protein identified in yeast, whereas seven Atg8 homolog proteins have been found in human cells (17, 18). During formation of the autophagosome, these Atg8 family proteins are first proteolytically processed by Atg4 to expose a C-terminal glycine (19). In...
B Participates with walking time less than 6 min in Gardner treadmill testing had higher levels of GRO and NAP2 protein. C The predict values of GRO and NAP2 for impaired walking capacity in Gardner treadmill testing. D Spearman correlation analysis between autophagy-related genes and walking ...
Expression of beclin-1, an autophagy-related protein, in gastric and colorectal cancers. APMIS, 2007, 115(12): 1344-1349. 22. Xia P, Wang JJ, Zhao BB, et al. The role of beclin-1 expression in patients with gastric cancer: a meta-analysis. Tumour Biol, 2013, 34(6): 3303-3307....
2007 . LC3, an autophagosome marker, can be incorporated into protein aggregates independent of autophagy: Caution in the interpretation of LC3 localization . Autophagy 3 : 323 – 328 .Kuma A, Matsui M, Mizushima N. LC3, an autophagosome marker, can be incorporated into protein aggregates ...
C, the protein lysates were subjected to a Western blot analysis of LC3 in cells pretreated with NAC. D, an analysis of the percentage of cells undergoing autophagy as detected by the GFP-LC3 assay with a fluorescent microscope in cells pretreated with NAC. The columns indicate the mean ...
Following protein denaturation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), the separated proteins were transferred to polyvinylidene fluoride (PVDF) membranes. Following transfer, membranes were incubated with corresponding primary antibodies at 4 °C overnight as follows: LC3 ...
13.Pingoud-Meier C, Lang D, Janss AJ, Rorke LB, Phillips PC, Shalaby T, Grotzer MA. Loss of caspase-8 protein expression correlates with unfavorable survival outcome in childhood medulloblastoma. Clin Cancer Res. 2003; 9:6401–9.[PubMed] ...
Apoptosis was enhanced as shown by breakdown of mitochondrial potential, up-regulation of cleaved caspase-3 and PARP-1 and down-regulation of anti-apoptotic protein Bcl-2. Formation of LC3-II complexes was inhibited by ethanol in caspase-3 deficient MCF-7 cells. Stimulation of autophagy by ...
CMA is a selective kind of autophagy, in which the protein constituents with a KFERQ-like pentapeptide motif are recognized by the cytosolic chaperone heat shock cognate protein (HSC70, also known as HSPA8) and directed to the lysosomal membrane surface by binding the motif to the cytosolic ...