当与 Hoechst 和溶酶体等核染料以及吖啶橙等细胞器标记物结合使用时,可以评估自噬水平。 5、 评估autophagic flux 因为自噬是一个动态过程,所以评估autophagic flux会比单纯评估自噬体数量更能反映此过程。 可以使用多种测定法检测autophagic flux。 通过比较不同条件下...
当与 Hoechst 和溶酶体等核染料以及吖啶橙等细胞器标记物结合使用时,可以评估自噬水平。 5、 评估autophagic flux 因为自噬是一个动态过程,所以评估autophagic flux会比单纯评估自噬体数量更能反映此过程。 可以使用多种测定法检测autophagic flux。 通过比较不同条件下或不同样本中的 LC3-II生成,可估计 LC3-II 更新...
we analyzed whether YTHDF3 regulates autophagy flux by simultaneously affecting lysosomal activity. Acridine orange (AO) is a cell-permeant dye that emits green fluorescence; however, when trapped in acidic compartments, AO emits red fluorescence when excited by...
Hence, Met-TKIs activated autophagy flux in Met-amplified GC cells. Fig. 1: Met tyrosine kinase inhibitors (Met-TKIs) induced autophagy in Met-amplified gastric cancer (GC) cells. a, b Met-amplified GC cells were treated with Met-TKIs as indicated and lysates were immunoblotted for proteins...
flux through autophagy pathways. For example, microtubule-associatedprotein 1light chain 3 (LC3) is lipidated from LC3-I to LC3-II and incorporated into the membranes of pre-autophagosomes for eventual degradation within the lysosome. LC3-II content is influenced by autophagic flux, but does not...
A bifluorescence autophagy system with the mRFP-GFP-LC3 plasmid was used to evaluate autophagy flux. Protein and mRNA levels were detected using Western blotting and qPCR, respectively. A Cell Counting Kit-8 (CCK-8) assay, colony assay, and flow cytometry analysis were used to evaluate cell ...
4e). Conversely, blockage of autophagic flux by bafilomycin A1 reverted the suppressive effect of VPS34 re-expression on micronuclei accumulation in TRABID-knockdown cells (Fig. 4e). In addition, the colocalization or encompassing of micronuclei with LC3, an indication of the micronucleophagy ...
Spermidine-enhanced autophagic flux improves cardiac dysfunction following myocardial infarction by targeting the AMPK/mTOR signalling pathway. Br. J. Pharmacol. 176, 3126–3142 (2019). CAS Google Scholar Gomes, A. P., Schild, T. & Blenis, J. Adding polyamine metabolism to the mTORC1 toolkit...
Previous study has shown that the adenosine monophosphate-activated protein kinase (AMPK)-mammalian target of rapamycin (mTOR)-transcription factor EB (TFEB) pathway serves as a novel regulator of cell fate determination during differentiation via TFEB-dependent regulation of autophagic flux [23]. Speci...
[6]Mauro-Lizcano M, Esteban-Martínez L, Seco E, Serrano-Puebla A, Garcia-Ledo L, Figueiredo-Pereira C, Vieira HL, Boya P.New method to assess mitophagy flux by flow cytometry. Autophagy. 2015;11(5):833-43. doi: 10.1080/15548627.2015.1034403IF: 13.3Q1. PMID: 25945953IF: 13.3Q1; PMCI...