Automated selection of aptamers against protein targets translated in vitro: from gene to aptamer. Nucleic Acids Res 30:e108 - JC, Hayhurst, et al. - 2002Cox, J. C., Hayhurst, A., Hesselberth, J., Bayer, T. S., Georgiou, G., and Ellington, A. D. (2002) Automated selection of...
Cell-free genetically encoded biosensors have been developed to detect small molecules and nucleic acids, but they have yet to be reliably engineered to detect proteins. Here we develop an automated platform to convert protein-binding RNA aptamers into riboswitch sensors that operate within low-cost ...