IPTG inductionLactoseProtein expressionAuto-induction media containing glucose, lactose, and glycerol are a simple and efficient approach for high-throughput protein expression in Escherichia coli with lac -derived expression systems. Its principle is based on inducer exclusion between glucose and lactose,...
This is the first report on utilizing CSL as auto-induction media for expression and purification of rhIFN-β-1b. Thus, the suggested method can be employed for simple as well as cost-effective production of rhIFN-β-1b and other recombinant proteins.Nasira Rafi...
Cells were cultured in LB broth with 50 µg/mL kanamycin at 37 ◦C to an OD600 of 0.5, then induced by 0.4 mM IPTG, and incubated overnight at 20 ◦C. The cells were lysed in Tris-NaCl buffer (20 mM Tris pH 7.0, 100 mM NaCl). The PltZ proteins with a N-terminal 6xHis...