The existence of an ATP-driven proton pump in plant plasma membranes was suggested two decades ago from physiological studies on active transport (Poole 1978) and by measurement of electrical potentials (Spanswick 1981) across the plasma membrane of whole plant cells (Chap. 8). The pioneering wor...
The plasma membrane proton-translocating adenosine triphosphatase (ATPase) is an archetype of the P-type ATPase family of membrane transporters, which are important in a wide variety of cellular processes. The H + -ATPase has been crystallized and its structure determined to a resolution of 8 ?
Protein kinase C and calmodulin effects on the plasma membrane Ca2+-ATPase from excitable and nonexcitable cells[J... D Kosk-Kosicka,L Zylińska - 《Molecular & Cellular Biochemistry》 被引量: 33发表: 1997年 A locally-induced increase in intracellular Ca2+ propagates cell-to-cell in the ...
The plasma membrane ATPase of plants and fungi is a hydrogen ion pump 1 . The proton gradient generated by the enzyme drives the active transport of nutrients by H + -symport. In addition, the external acidification in plants and the internal alkalinization in fungi, both resulting from acti...
+-ATPase Reconstituted into Nanodiscs Is a Monomer.]]>doi:10.1074/jbc.M112.446948Plasma membrane H+-ATPases form a subfamily of P-type ATPases responsible for pumping protons out of cells and are essential for establishing and maintaining the crucial trans-membrane proton gradient in plants and...
Plant plasma membrane H(+)-ATPase acts as a primary transporter via proton pumping and regulates diverse physiological responses by controlling secondary solute transport, pH homeostasis, and membrane potential. Phosphorylation of the penultimate threonine and the subsequent binding of 14-3-3 proteins in...
2 Plasma membrane-localized RAC1 is required for RAS-induced macropinocytosis. a, b, Effect of V1A expression on RAC1 activity. a, Immunoblots of RAC1 wild type and RAC1(L61) activity (GST–PBD, pull-down of GFP–RAC1–GTP; vinculin loading control) with or without V1A knockdown in...
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The role of the plant plasma membrane H+-ATPase in K+ uptake was examined using red beet (Beta vulgaris L.) plasma membrane vesicles and a partially purified preparation of the red beet plasma membrane H+-ATPase reconstituted in proteoliposomes and planar bilayers. For plasma membrane vesicles,...
THE INSECT V-ATPase, A PLASMA MEMBRANE PROTON PUMP ENERGIZING SECONDARY ACTIVE TRANSPORT: IMMUNOLOGICAL EVIDENCE FOR THE OCCURRENCE OF A V-ATPase IN INSECT... Active electrogenic K+ in serves as the energy source for or absorption in gastrointestinal epithelia or for the receptor current in sensory...