Box 653, Beer Sheva, IsraelJohn Wiley & Sons, Ltd.FEBS JournalShoshan V and Shavit N (1979) ATP synthesis and hydrolysis in chloroplast membranes. Differential inhibition by antibodies to chloroplast coupling factor 1. Eur. J. Biochem. 94, 87–92....
Lettura consigliata Tags Hydrolysis ATP Adenosine Triphosphate Energy Storage Phosphate Groups Water Addition Exergonic Process ADP Adenosine Diphosphate Inorganic Phosphate AMP Adenosine Monophosphate Bonds Cell Energy Use Protein Synthesis JoVE Privacy • Terms of Use • Policies Share on Twitter Share...
A Possible Mechanism for ATP Synthesis and ATP Hydrolysis by the FOF1 ATPase of ChloroplastsOn FOF1 ATPases, three catalytic nucleotide sites with different properties were found. Following hypotheses for ATP synthesis (1,2) these properties change for each site in an alternating or a cyclic ...
and results in the synthesis/hydrolysis of ATP. It is not the synthesis of ATP but its release from the synthase that is driven by the protonmotive force. Subunitsγandɛrotate relative to theα3β3hexamer to alter the nucleotide affinities (and hence binding) in theβ-catalytic sites. ...
(ATP5B) form a hexamer with six ADP/ATP binding sites, among which only the β subunits are capable of catalyzing ATP synthesis/hydrolysis while the α subunits are catalytically inactive34,35. As ATP5B has been identified as a Cr3+-binding protein both in vitro and in cellulo, we ...
Since the main function of the ATP synthase is the production instead of hydrolysis of ATP, the most interesting measurements are those in synthesis direction. Such experiments were performed with yeast ATP synthase reconstituted into vesicles and the ATP synthesis rate and ATP yield was compared as...
The two terminal ester bonds (β and γ) are more energy rich. The energy released from hydrolysis of the γ-ester bond can be used in a wide range of biological system, and therefore ATP is also known as the energy currency. Although ATP can been synthesized through substrate level ...
F1Fo-ATP synthase not only synthesizes ATP but is also solely responsible for 80–90% of mitochondrial ATP hydrolytic activity17. The intracellular ATP content is determined by the rates of ATP synthesis and ATP hydrolysis18. Deletion of the δ subunit impairs ATP synthesis through OXPHOS, and ...
This step is divided into sub-steps of 80° and 40°, triggered by ATP binding and by hydrolysis or product release, respectively [5, 8]. However, much less is known about ATP synthesis. In a recent study, Rondelez et al. [9] made accurate quantitative measurements of both ATP ...
IF1 binds to the alpha(DP)-beta(DP) interface of the F1FO ATP synthase at the matrix side and contacts the central gamma subunit [28,29] preventing futile hydrolysis of ATP [30]. The inhibitory peptide dimerizes and oligomerizes in a pH-dependent manner, for which a protonated histidine ...