For DNA digestion of single and double-stranded DNA, 1 μg RNA sample was incubated at room temperature for 10 min with 1 μl 10× DNase I Reaction Buffer, 1 μl DNase I Amp Grade 1 U/μl, and DEPC-treated water to final volume of 10 μl. DNase I was inactivated by incubation ...
Adenosine triphosphate (ATP) plays fundamental roles in cellular biochemistry and was recently discovered to function as a biological hydrotrope. Here, we use mass spectrometry to interrogate ATP-mediated regulation of protein thermal stability and prote
The proteins were digested by adding a solution of trypsin in 50 mM NH4HCO3 (20 ng/μl sequence grade, Promega). The mixture was incubated for 3 h at 37 °C. 2.18. Nanoelectrospray tandem mass spectrometry Recovered peptides were subjected to nanoLC-MS/MS analysis on an ESI-Q-TOF mass...
An analysis was performed to determine the correlation between T2 MRI values in endometrial cancer (EC) and pathological characteristics, including International Federation of Gynecology and Obstetrics (FIGO) stage and grade.Middling T2 values for NE, BEL, and EC were found to be 1975 ms (ranging...
All other chemicals were of the highest grade from commercial sources 2.7. Statistical analysis Values obtained in this study are expressed as mean±S.E. Statistical comparison was made by one-factor analysis of variance or the Student–Newman–Keuls procedure to test for significant differences. ...