Fluids and Barriers of the CNS volume 21, Article number: 62 (2024) Cite this article 967 Accesses Metrics details Abstract Background Pharmacotherapy for brain diseases is severely compromised by the blood-brain barrier (BBB). ABCB1 and ABCG2 are drug transporters that restrict drug entry into...
For DNA digestion of single and double-stranded DNA, 1 μg RNA sample was incubated at room temperature for 10 min with 1 μl 10× DNase I Reaction Buffer, 1 μl DNase I Amp Grade 1 U/μl, and DEPC-treated water to final volume of 10 μl. DNase I was inactivated by incubation ...
(v/v) for 5 h followed by pure medium-grade acrylic LR White resin (Agar Scientific,http://www.agarscientific.com) for 2 days, taking care to replace the resin every day. Samples were finally placed in the center of capsules and placed in a heat chamber for 24 h (67 °C). ...