In this study, however, CVA6 procapsids and A-particles can be obtained by routine methods of continuous, sucrose density gradient ultracentrifugation. CVA6 A-particles isolated and purified in this manner exhibit the biochemical composition and structural features similar to the A-particles of ...
The first two ingredients were sucrose and corn syrup. The Space Food Sticks were available to the astronauts starting with Apollo 11, and were designed to do exactly what the government asked from Pillsbury: provide contingency food when the astronauts were confined to their pressurized spacesuits...
After cell debris was removed, the virus containing supernatant was subjected to ultracentrifugation on a 20% sucrose cushion (SW28 Ti rotor, 131,000g, 4 °C, 3 h) and then on a 10–50% sucrose gradient (SW60 Ti rotor, 205,000g, 4 °C, 2.5 h) in a Beckman centrifuge....
increasing the efficiency will bring the break-even duration point lower. The mass of the CELSS is constant regardless of mission duration, the mass of consumables goes up with mission duration.