a, Dependency of Seurat label transfer scores and average log2 (fold change) of the top 2,000 DARs in selected cell types on the total number of cells in selected cell types.b,c, Heat map of the number of DARs (b) and heat map of the fold change enrichment (c) of the top 2,...
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Skeletal muscle development is a multistep process whose understanding is central in a broad range of fields and applications, from the potential medical value to human society, to its economic value associated with improvement of agricultural animals. Skeletal muscle initiates in the somites, with mu...
Each tissue for scRNA-seq was dissociated in 500 μL dissociation agent (400 U/mL DNaseI on hibernate E buffer, 10 U/mL papain) at 37°C on a thermocycler for 15 min. Dissociation was terminated by 500 μL 10% FBS in Hibernate E buffer. Cells were centrifuged at 4°C ...