An 'Asymmetry Factor' refers to a measure of peak asymmetry in chromatography, calculated by dividing the width segment after the peak maximum projection by the width segment before the peak maximum projection. A symmetrical peak has an Asymmetry Factor of 1.0, with values between 0.9 and 1.2 co...
The analyses of peak shapes in chromatography are useful in operating chromatographic system. The asymmetry and sharpness of a chromatographic peak are estimated by the reversed-phase adsorption of two standard peptides (angiotensin II bradykinin) on C18. In this work, the average particle diameters ...
post-processing in RELION. Optimal B-factor sharpening values were determined empirically. B-factors of −75 Å2for the Fab-proximal domain and −50 Å2for the Fab-distal resulted in the best combination of map detail and continuity of density. The resulting maps were combined in Chi...
Two simple graphical approaches are presented to analyze peak shapes typically observed in gas, liquid, and supercritical fluid chromatography as well as capillary electrophoresis. The derivative test relies on the symmetry of the inflection points and the maximum and minimum values of the derivative. ...
The asymmetry in burial of hydrophobic residues along the lengths of the histone chains of these structures was investigated as well as sequence and structural homology with other archaeal and eukaryotic, nucleosomal and transcription factor histones. Whereas previous studies have emphasized structural ...
Eluted A2AAR was concentrated to 500 ml in a Vivaspin 6 mL concentrator with a 100,000 molecular weight cutoff and further purified by size exclusion chromatography using a Superdex 10 ×300 column with buffer containing 25 mM HEPES, pH 7.5, 150 NaCl, 0.025% DDM/CHS, and 100 ...
(galactose-responsive transcription factor, able to constitutively activate transcription), were plated on SD –Leu plates and were the positive control; Y190 cells transformed with pGBKT7-thraaand pGADT7 or with pGBKT7-thrb1and pGADT7 were plated on SD –Leu–Trp plates; Y190 cells ...
hm A, Jenal U, Seifert R, Kaever V (2010) A liquid chromatography-coupled tandem mass spectrometry method for quantitation of cyclic di-guanosine monophosphate. J Microbiol Methods 81: 226–231 doi:10.1016/j.mimet.2010.03.020. [32] Ravenscroft N, Walker SG, Dutton GG, Smit J (1991) ...
Detection of RA concentration by ultraperformance liquid chromatography coupled to tandem mass spectrometry (UPLC–MS/MS). Skin was collected from the ocular and blind sides of post-metamorphic flounder juveniles (75 DAH). Skin (0.1 g) was ground in liquid nitrogen and transferred to glass centrif...
It might very well be an important factor, but another at present unknown factor also seems to play an important role in the events that will lead to the exposure of PS, preferentially localized to areas that seemed to bulge over an inclusion body. In conclusion, we have shown that in ...