They minimally exhibit a β,γ-didomain architecture that has been evolutionarily repurposed in a wide range of terpene-processing enzymes and likely resulted from a fusion of unidentified monodomain proteins. Although single domain class I terpene cyclases have already been identified, the ...
Specific uS5-associated proteins were determined by a quantitative proteomics approach, stable isotope labeling by amino acids in cell culture (SILAC), that classifies interactions on the basis of specificity (ratio of peptide intensities between the GFP–uS5 pulldown and the control purification) ...
These interactions and bound proteins determine the structure of chromatin and its capacity for being transcribed. The chromatin-associated proteins recognize a particular histone marking and might have the ability to generate the same mark Dhalluin et al. 1999, Jacobson et al. 2000, Lachner et ...
The protease fold of CtpB is directly related to the cage-forming ClpP and tricorn proteases, which function as molecular shredders in bacterial protein quality control. For both cage-forming proteins, the proteolytic sites are buried within a self-compartmentalizing particle, in which substrate filt...
This enzyme, Cgc3∗, belongs to the AMP-binding superfamily of proteins and resembles acyl-CoA synthetases. One of Cgc18_A and Cgc3∗ probably acts iteratively to activate the two pyrrole precursors, the other enzyme activating guanidinoacetate. The amino acids identified by established models...
The glutathione S-transferase (GST)-IMPDH1 WT and GST-IMPDH1 (deletion of 108–245 amino acids [aa]) with FLAG-YB-1 proteins were purified for GST-pull-down and western blot assays to examine the direct binding of IMPDH1 and YB-1 proteins in vitro. As shown in Figure 5C, the ...
RNA helicases like Vasa are ATP-gated RNA-binding proteins that engage in dynamic associations. We used a point mutation (DQAD) within the DEAD box of Vasa to prevent release of ATP hydrolysis products, locking the protein onto its bound RNA targets and reducing its in vivo dynamics. ...