Means of responses of log10 data are reported for each participant (6 participants, 54 donors, time 24 h; 1 participant, 8 donors, time 48 h). p values for the comparison of each positive control with its isotype control are reported in supplementary table 2. The two main CRA methods...
The T/C value gradually increased as the abrin concentrations increased from 0.1 to 1000 ng mL−1, which provides the basis for abrin quantitation. A standard curve and the quantitative equation are shown in Fig. 3c, with the logarithm of the T/C–cut-off value as x and the ...
The new assay, which is based on a suspension-array hybridization method, was tested on a series of male and female reference samples spanning the range from normal to full mutations. It was also tested on DNA from 1008 dried blood spot samples from pregnant women in their first trimester. ...
First, 5 μL PSA was coated on the working electrodes at 37 °C for 1 h. Then, 5 μL of 10 μg/mL HRP-Ab2 was added to the electrode surface at 37 °C for 1 h, and redundant reagents were washed away with PBS and dried under N2. Finally, each electrode was ...
A problem with the term borderline diabetes was that its definition was not uniform, which was partly due to inconsistencies in the procedure of performing a glucose tolerance test, with the amount of glucose ingested varying from 50 to 100 g or given on a kilogram basis. IGT as an entity ...
5A). On the basis of our previous work (2) and the data presented in the present article, we conclude that PAPC plus HPODE generated less Ox-PAPC in the presenceof vitamin E or lutein. However, analogous to the case for the coculture assay (11), both vitamin E and lutein failed ...
These soils were selected for evaluation on the basis that they contained minimal levels of “unknown” precursors (Liu et al., 2022). This level was previously determined by comparing the levels of quantitative and semi-quantitative compounds to the heat-activated TOP assay results. However, Fig...
Jameson P, Greiff D, Grossberg SE (1979) Thermal stability of freeze-dried mammalian interferons. Analysis of freeze-drying conditions and accelerated storage tests for murine interferon. Cryobiology 16:301–314PubMedView Article Johnston MD, Finter NB, Young PA (1981) Dye uptake method for assay...
Next, the alpha and beta compounds tethered to the solid support and the first cleavage means are added to the growth media, forming a growth media complex. The method further comprises incubating the growth media complex, during which the microorganism grows within and on the growth media and ...
(Developmental Studies Hybridoma Bank, University of Iowa), for an additional 1 h on ice. Samples were then subjected to 4% (w/v) polyacrylamide gel electrophoresis in 0.5 Tris/borate/EDTA buffer. After electrophoresis, the gels were dried and exposed to Kodak film at 70° C. for 12-48 ...