A method for validating an immunohistochemical slide. The slide is pre-treated and stained in accordance with an immunohistochemical protocol and includes a validation portion with at least one control group. The at least one control group is configured to validate at least one parameter directed to...
Five CSF samples were diluted 1:2 in assay buffer and then further diluted in twofold steps until a final dilution of 1:16. All dilutions and undiluted samples were subjected to the assay protocol and analyzed in triplicate. Parallelism assesses the similarity between the dose–response curves o...
this case the validation goal might be tocharacterizethe method, using the validation results to establish an assay format that is predicted to yield reliable product measurements. A sound justification for target acceptance criteria or use ofcharacterizationshould be included in the validation protocol....
The following thermocycling protocol was used for real-time PCR, on an Applied Biosystems™ 7500 Real-Time PCR System (Applied Biosystems, Waltham, MA): (1) an initial denaturation step of 3 min at 95 °C and (2) 50 cycles of 15 s at 95 °C and 30 s at 60 °C, with a fluor...
The MPCVA method predicted that cells treated with UV, H2O2, and TBBPA had the lowest viabilities, whereas starved cells and cells treated with 5 FU had the highest viabilities (Figure 10a). The addition of cell count measurement to the protocol increased the performance of the assay, ...
SRM have been used for theanalysis of proteinsand peptides in body fluids such as blood plasma/serum[154–157]or urine[158,159]. It has been successfully applied to the analysis of Hb variants relevant in hemoglobinopathies[154,155]. Moat et al. developed a protocol for new-born blood ...
These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing ...
We used a high-throughput comet protocol with fully automated scoring of DNA strand break levels. The overall results from fresh and frozen samples were in agreement [R2 = 0.93 for %DNA in tail (%TDNA) and R2 = 0.78 for tail length (TL)]. A slightly increased %TDNA was observed in ...
Statistical validation of 1H NMR protocol vs standard biochemical assay in quality control of RBC packed units BACKGROUND: Time dependent quantification of endogenous metabolites in biological samples (blood, urine, biological tissues extracts) in normal and patholo... T A. Pertinhez,E Casali,L Zamb...
The latter protocol has been optimized to allow an increased throughput, as potential applications require large numbers of samples. Both approaches can be completed in a day. 展开 关键词: Humans Mitogen-Activated Protein Kinase 14 Proteins Drug Delivery Systems Blotting, Western Protein Binding MAP ...