The signal from a mole of GSSG will appear as the signal from two moles of GSH; therefore, the measured value for the oxidized glutathione is divided by two. Sample Calculation Total Glutathione Data Sample Net RLU Vehicle Control 14,989,339 Treated 12,364,719 GSSG Data Sample Vehicle ...
as demonstrated by a simple model calculation by Schluter et al.[181]. Nevertheless a SRM assay for quantification of a protein species is applicable, if it is possible to detect it's unique tryptic peptide by SRM. E.g. Wang et al. determined the ratio of peptides coded by normal and ...
5E were quantified using ImageJ (https://imagej.net/ij/) and used for the calculation of putrescine (Spe3-mediated conversion rate) and spermidine consumption rates (Spe4-mediated conversion rate) (Fig. S10, A–B). The levels of spermidine determined using the DAB-APT assay were similar ...
Cells may be left in medium at this point at room temperature in the dark for 1–2 h with the tubes sealed. Culture of lymphocytes, addition of Cyt-B and harvesting of cells Timing 72 h 14 Using the above calculation (Step 13), resuspend the cells at 1 × 106 per ml into the cu...
‘Dilution Factor’ in the assay parameter in macrofile ‘Neutralization_SARS-CoV-2’ to 1/3 (threefold serial dilution) before calculation. The concentration decreases threefold with the increase of one dilution. The ‘Start Dilution’ should be changed to the initial concentration of the ...
A conservative approach was adopted, assuming a POCT sensitivity lower than that both declared by the manu- facturer [33] and proposed by WHO target product pro- files for NG and CT POCTs [34]. The formula used for the sample size calculation, based on the 2006 WHO/ TDR expert panel ...
.Determine the absorbances of the solutions in 5-cm cells at the wavelength of maximum absorbance at about 530nm,with a suitable spectrophotometer,using the blank to set the instrument.Calculate the quantity,in mg,of epinephrine (C9H13NO3)in each mLof the Injection taken by the formula:...
In another studies, the nature of the interaction (synergy, additivity, or antagonism) between an antimicrobial drug and a natural compound is obtained with the median-effect method of Chou (2006, 2010) that allows the calculation of the combination index (CI). For two-drugs combination, both...
This calculation has been summarised in Fig. 3b. Western blot module To find the oxygen concentration at which HIF1α is expressed, the MCF7 cell line was cultivated in-vitro at different oxygen concentrations (1, 5, and 21%) and then Western blots [24] were run for HIF1α. Standard ...
See more details about the calculation of input/output variables in the next sections. The objective of the IFPTML model is to predict a function f(vij)calc of the observed values f(vij)obs. In order to develop the IFPTML model, we took into consideration both structural and functional ...