aspartic acidisoaspartic acidkineticsstabilitycapillary electrophoresisThe aim of the present study was the determination of the isomerization and enantiomerization of aspartic acid (Asp) in tripeptides. Capillary electrophoresis (CE) assays were developed and validated allowing the simultaneous determination ...
In particular, isoAsp was significantly elevated at these sites in MS patients. The generation of isoAsp could be responsible for eliciting an immune response to modified MBP and therefore be implicated in the etiology of MS. 展开 关键词: ISOASPARTIC acid MULTIPLE sclerosis MYELIN sheath ...
This heterodimer, like all members of the “v” integrin family, recognizes the amino acid motif Arg-Gly-Asp (RGD), present in a variety of bone-residing proteins such as osteopontin and bone sialoprotein. Thus, osteoclasts attach to, and spread on, these substrates in an RGD-de...
Isomerization of aspartic acid (Asp) in therapeutic proteins could lead to safety and efficacy concerns. Thus, accurate quantitation of various Asp isomerization along with kinetic understanding of the variant formations is needed to ensure optimal process development and sufficient product quality control...
Aspartic Acid Isomerization Characterized by High Definition Mass Spectrometry Significantly Alters the Bioactivity of a Novel Toxin from Poecilotheriadoi:10.3390/TOXINS12040207Stephen R JohnsonHillary G RikliToxins (Basel)
report the implementation of capillary zone electrophoresis coupled to electrospray ionization tandem mass spectrometry (CZE-ESI-MS/MS) by the intermediate of a sheathless interfacing for independent and highly sensitive characterization of asparagine deamidation (deaN) and aspartic acid isomerization (isoD...
Isomerization of an aspartic acid residue in the complementarity-determining regions of a recombinant antibody to hu- man IgE: identification and effect on binding affinity. Biochemistry. 1996;35(6): 1897-1903.Cacia, J.; Keck, R.; Presta, L.G.; Frenz, J. Isomerization of an aspartic acid...
The isomerization rate of aspartic acid (Asp) is high and causes various serious diseases including Alzheimer's disease and cataract. Herein, a method for the comprehensive separation and sensitive detection of isomerized crystallin containing Asp (l-α-Asp, l-β-Asp, d-α-Asp, and d-β-Asp...
Fumaric acid is produced by reacting a culture of a microorganism which produces maleate isomerase that exhibits a maximum activity at not less than 50掳 C. or a treated product thereof with maleic acid in an aqueous solution, and isomerizing maleic acid to produce fumaric acid. L-aspartic ...
High level of aspartic acid-bond isomerization during the synthesis of an N-linked tau glycopeptide. J. Pept. Sci. 1999; 5:442–456.Hoffmann R, Craik DJ, Bokonyi K, Varga I, Otvos L Jr (1999) High level of aspartic acid-bond isomerization during the syn- thesis of an N-linked tau...