aspartic acidisoaspartic acidkineticsstabilitycapillary electrophoresisThe aim of the present study was the determination of the isomerization and enantiomerization of aspartic acid (Asp) in tripeptides. Capillary electrophoresis (CE) assays were developed and validated allowing the simultaneous determination ...
This heterodimer, like all members of the “v” integrin family, recognizes the amino acid motif Arg-Gly-Asp (RGD), present in a variety of bone-residing proteins such as osteopontin and bone sialoprotein. Thus, osteoclasts attach to, and spread on, these substrates in an RGD-dep...
Isomerization of aspartic acid (Asp) in therapeutic proteins could lead to safety and efficacy concerns. Thus, accurate quantitation of various Asp isomerization along with kinetic understanding of the variant formations is needed to ensure optimal process development and sufficient product quality control...
Mass spectrometric quantitative studies of modifications, which do not change the mass of the protein, such as isomerization of aspartic acid, do not necessarily require the use of isotope-labelled standards. However, the accurate solution of this problem requires a deep understanding of the ...
High level of aspartic acid-bond isomerization during the synthesis of an N-linked tau glycopeptide. J. Pept. Sci. 1999; 5:442–456.Hoffmann R, Craik DJ, Bokonyi K, Varga I, Otvos L Jr (1999) High level of aspartic acid-bond isomerization during the syn- thesis of an N-linked tau...
Isomerization or maleic acid,maleate isomerase,also production of aspartic acid. Goto M,Nara T,Tokumaru I,et al. U.S.Patent 5,783,428 . 1998US5783428 * 1997年2月20日 1998年7月21日 Mitsubishi Chemical Corporation Isomerization or maleic acid, maleate isomerase, also production of aspartic ...
High level of aspartic acid-bond isomerization during the synthesis of an N-linked tau glycopeptide. J. Pept. Sci. 1999; 5:442–456.Hoffmann, R., Craik, D.J., Bokonyi, K., et al. High level of aspartic acid-bond isomerization during the synthesis of an N-linked tau glycopeptide....
Aspartic Acid Isomerization Characterized by High Definition Mass Spectrometry Significantly Alters the Bioactivity of a Novel Toxin from Poecilotheriadoi:10.3390/TOXINS12040207Stephen R JohnsonHillary G RikliToxins (Basel)
The diagnostic ion c6 + 57 was observed in the ECD spectra of the Aβ1-42 peptide, demonstrating isomerization at residue 7. In conclusion, both ECD and EID can clearly determine the presence and the position of isoaspartic acid residues in amyloid β peptides. The next step, therefore, ...
The isomerization rate of aspartic acid (Asp) is high and causes various serious diseases including Alzheimer's disease and cataract. Herein, a method for the comprehensive separation and sensitive detection of isomerized crystallin containing Asp (l-α-Asp, l-β-Asp, d-α-Asp, and d-β-Asp...