to a pyroglutamyl residue when it is deprotected and exposed to weak acids such as HOBt or the Boc-amino acid of the next coupling step (DiMarchiet al., 1982). The coupling to Gln with a nonacidic species such as an active ester or preformed symmetrical anhydride was therefore ...
(Asn) in position B3 of the B chain is replaced by a naturally occurring basic amino acid residue and at least one amino acid residue in the positions B27, B28 or B29 of the B chain is replaced by another naturally occurring neutral or acidic amino acid residue, where phenylalanine (Phe...
To examine whether asparagine preferentially exchanges with certain amino acids, asparagine- pre-loaded LPS2 parental cells were treated with non-polar, basic or serine/threonine (which share a transporter) amino acid sub- categories. Although asparagine export is observed with all three groups, ...
4a). In addition, following a 5-min amino acid stimulation, intracellular non-polar and basic amino acid levels are approximately 50% higher in asparagine-pre-loaded glutamine-independent LPS2 cells compared with cells that lack a pre-load, while intracellular serine and threonine levels are ∼...
Deamidation rate at 40°C was faster in acidic buffers than in basic buffers. However, this trend is reversed at 5°C, attributed to the change in hydroxide ion concentration influenced by buffer and temperature. The apparent activation energy was higher for rates generated in an acidic buffer ...
The main p31 isoform and the splicing variant p41 form of the Ii chain were digested to produce a discrete pattern of bands at mildly acidic pH (e.g., Figure 1A, track 2) whereas the α and β chains of MHC class II were comparatively resistant. We then used various protease inhibitors...
The incorporated radiolabel was stable over long periods of time and at acidic or basic pH as long as elevated temperatures were avoided. The radiolabel was removed by treatment of the protein at pH 10 and 50 degrees C or with 0.2 M hydroxylamine at 50 degrees C for at least 30 min. ...
Why would they be considered nonpolar, polar, acidic, or basic? What is an essential amino acid? What is the name of the enzyme that attaches amino acid to the tRNA? Draw a structural formula of simple amino acid, and identify the carboxyl group...
Although it shows a similar folding pattern to the propeptides of other papain family members[31,32]the cathepsin B pro region is relatively short. The isolated propeptide is a potent, selective, cathepsin B inhibitor[33]but the dissociation constant increases dramatically under acidic conditions. ...
VyPAL2 is an asparaginyl ligase fromViola yedoensis, which has a broad specificity for the C-terminal motif. In one example, Liu et al. exploited the different activity of the enzyme for Asp or Asn motifs at acidic and neutral pH to perform dual labeling of GFP and affibodies in the C-...