The ratepH profile for mAb1 can be deconvoluted to Fc and Fab. The Fc deamidation showed a V-shaped profile: deamidation of PENNY peptide is responsible for the rate at high-pH, whereas deamidation of a new site, Asn323, may be responsible for the rate at low-pH. The profile for Fab...
图:1/2 Asparagine Synthetase Antibody (PA5-29973) in IHC (P) Immunohistochemical analysis of paraffin-embedded U87 xenograft, using Asparagine synthetase (Product # PA5-29973) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.View more ...
cells [94], largely explaining the decrease in asparagine levels in replicatively aged yeast [95]. Asparagine supplementation only slightly extended mean lifespan inC. elegansat a 5 mM dose and decreased lifespan to the greatest extent of any amino acid at a 10 mM dose (Table 1) [3]...
HS Code29241900 ASPARAGINE H2O -Reference Information biological activityL-asparagine is a non-essential amino acid involved in the metabolic regulation of nerve and brain cell function, suitable for a variety of pharmaceutical applications. Useis a pharmaceutical intermediate, can also be used in bioch...
For experiments involving glutamine and asparagine pre-loading, serum- and amino acid-starved cells were incubated with 2 mM glutamine, 2 mM asparagine or 2 mM asparagine and 2 mM glutamine in starve medium for 1 h at 37 °C. Pre-loaded cells were washed twice with PBS to remove residual...
Slides were autoclaved at 120 °C for 10 min in Target Retrieval Solution (pH 6.0; Dako, Glostrup, Denmark) after deparaffinization using Fast Solve. Quenching was performed using 3% hydro- gen peroxide (H2O2) in methanol for 20 min, followed by blocking with 5% skim milk in ...
The ratepH profile for mAb1 can be deconvoluted to Fc and Fab. The Fc deamidation showed a V-shaped profile: deamidation of PENNY peptide is responsible for the rate at high-pH, whereas deamidation of a new site, Asn323, may be responsible for the rate at low-pH. The profile for Fab...
pka2.17(at 20℃) formPowder colorWhite PH5.41 Odorsltly sweet taste optical activity-5.625 (H2O)+28.625 (1 mol dm-3 HCl) Water SolubilityH2O: 20 g/L (20 oC) , clear, colorless SensitiveHygroscopic Merck14,837 BRN1723527 Stability:Stable, but may be moisture-sensitive. Incompatible with st...
Radioactive assays were performed in a 25 ml reaction volume containing 20 mM Tris pH 8.0, 20 mM NaCl, 110mmCiMofA[TUP-1, 41C0]m-aMspaMrtagtCel2(,2050mmMCgi lmutmamoliÀne1,).0A.5fmtegr of recombinant PbAS and incubating at 37 °C for 1 h, the assays were terminated by ...
Blood asparagine concentration is much lower at 0.05–0.1 mM (ref. 27), and media asparagine concentration ranges from 0 mM in DMEM to 0.38 mM in RPMI. Moreover, we found that intracellular glutamine levels are 10–100-fold higher than asparagine levels, depending on the cell line...